We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for the measurement of a single analyte. In our approach, devices are designed to simultaneously quantify analytes using multiple indicato...

EXTENDED ABSTRACT Biosensors provide a sensitive, specific, rapid, portable, and inexpensive means to fulfill the need for rapid on-site determinations of hazardous pollutants (e.g., explosives). Recent advances in instrumentation at NRL have led to two methods for measuring TNT and RDX: the FAST 2000 and the Analyte 2000. Engineering and design of both instruments was performed by Research Int...

We design a novel nano-ring resonator using two-dimensional photonic crystal (2D-PhC), for bio-sensing applications. The structure of biosensor is created by two-curve-shaped ring resonator which sandwiched by two waveguides. These are configured by removing one row of air holes. The refractive index of sensing hole is changed by binding an analyte. Hence, intensity of the transmission spectrum...

The electrodeposition of gold nanostructures increases the surface area of a biosensor, which brings an enhancement of the sensitivity by increasing the amount of analyte binding to the surface. To evaluate the relationship among the surface structure, the area and the analyte binding, we quantitatively analyzed them for quartz crystal microbalance (QCM) sensing by scanning electron microscopy...

A new fluorescence-based biosensor is presented. The biosensing scheme is based on the fact that a fluorophore in close proximity to a metal film (<100 A) experiences strong quenching of fluorescence and a dramatic reduction in the lifetime of the excited state. By immobilizing the analyte of interest (or a structural analog of the analyte) to a metal surface and exposing it to a labeled recept...

Three antituberculosis medications are investigated in this work consist of rifampicin, isoniazid and pyrazinamide. The ultra violet (UV) spectra of these compounds are overlapped, thus use of suitable chemometric methods are helpful for simultaneous spectrophotometric determination of them. A generalized version of net analyte signal standard addition method (GNASSAM) was used for determinatio...

In label-free biomolecular interaction analysis, a standard injection provides an injection of uniform analyte concentration. An alternative approach exploiting Taylor dispersion produces a continuous analyte titration allowing a full analyte dose response to be recorded in a single injection. The enhanced biophysical characterization that is possible with this new technique is demonstrated usi...