Objectives: To evaluate the amnion graft in vaginoplasty, to assess the advantage of a flap placed over fourchette and to evaluate the benefit of gauze mould. Methods: Out of the 35 subjects of primary amenorrhea, eight with complete vaginal agenesis were selected for vaginoplasty. Half of these received split thickness skin graft and other half received amnion graft. An additional triangular f...

Background: Split-thickness skin graft (STSG) covers patient’s primary wound, but, at the expense of a donor-wound which heals by secondary intention. This study evaluated efficacy amnion and calcium alginate dressings STSG donor sites in terms healing duration, patient comfort incidence infection.
 Patients methods: An open label randomised control trial was conducted during October 2018 ...

OBJECTIVE The proteoglycan decorin stabilizes collagen whereas biglycan and hyaluronan disrupt well-organized collagen. The aim was to compare hyaluronan and proteoglycans in human fetal membranes obtained before and after spontaneous labour at term. STUDY DESIGN Prelabour samples of fetal membranes (N=9) were obtained from elective caesarean sections and regionally sampled from over the cerv...

Analysis by means of two-dimensional gel electrophoresis (IEF) of [32P]orthophosphate-labeled proteins from mitotic and interphase transformed amnion cells (AMA) has shown that keratins IEF 31 (Mr = 50,000; Hela protein catalogue number), 36 (Mr = 48,500), 44 (Mr = 44,000), 46 (Mr = 43,500), as well as vimentin (IEF 26; Mr = 54,000) are phosphorylated above their interphase level during mitosis...

Cells of FL strain and primary human amnion cultures have been exposed to high hydrostatic pressure, and their ultrastructure has been observed following pressure release. Pressure treatment reveals a high degree of intercellular adhesiveness in primary amnion characterized by the formation of intercellular "bridges." Electron micrographs of these cells reveal well defined desmosomes associated...

Fetal membranes usually rupture during the process of labor. Premature fetal membrane rupture occurs not infrequently and is associated with significant fetal and maternal morbidity. The mechanisms of normal and pathologic fetal membrane rupture are not well understood. We have examined structural and biochemical changes in the rat amnion as labor approaches in order to characterize this proces...

Human cytomegalovirus (CMV) is the most common cause of viral intrauterine infection in developed countries. It has been shown that CMV DNA was frequently detected in the fetal membranes when the placenta was infected in utero. However, it is still not clear whether CMV replicates in constituent cells of the fetal membranes. We investigated CMV infection of primary cultured chorion and amnion c...

In the present study, we investigated the ability of human fetal membranes (amnion and choriodecidua) to regulate human maternal uterine cell functions through the secretion of surfactant protein (SP)-A and SP-D at the end of pregnancy. We detected the expression of both SP-A (SP-A1 and SP-A2) and SP-D by quantitative reverse transcription polymerase chain reaction. Immunohistochemistry reveale...

Interstitial collagen gives fetal membranes tensile strength, and membrane rupture has been attributed to collagen degradation. A polymorphism at -1607 in the matrix metalloproteinase-1 (MMP-1) promoter (an insertion of a guanine (G)) creates a core Ets binding site and increases promoter activity. We investigated whether this polymorphism is functionally significant for MMP-1 expression in amn...

Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from...