Total mitochondrial aspartate aminotransferase (EC 2.6.1.1), the sum of apo- and holo-mitochondrial aspartate aminotransferase activity in human serum, was measured by using a proteolytic method: inactivation of cytosolic aspartate aminotransferase with cytosolic aspartate aminotransferase-inactivating protease 401 from Streptomyces violaceochromogenes. Cytosolic aspartate aminotransferase is c...

The activity of aspartate aminotransferase (AST) in human serum has been widely determined as a diagnostic aid in liver disease. In this study, the effect of aluminium on AST isoenzymes in relation to aluminium intoxified patients has been investigated. Using gel filtration chromatography technique with Sephacryl S-300, mitochondrial aminotransferase (m-AST) and cytosolic aminotransferase (c-AS...

The subcellular distribution of asparagine:oxo-acid aminotransferase (EC 2.6.1.14) in rat liver was examined by centrifugation in a sucrose density gradient. About 30% of the homogenate activity after the removal of the nuclear fraction was recovered in the peroxisomes, about 56% in the mitochondria, and the remainder in the soluble fraction from broken peroxisomes. The mitochondrial asparagine...

The inner mitochondrial membranes from bovine heart, rat liver, and Morris hepatoma 7777 all bound the mitochondrial isozymes of aspartate aminotransferase and malate dehydrogenase with comparable affinities and binding ratios (mg of enzyme bound per mg of membrane protein). A low molecular weight fraction separated from a detergent extract of the heart membrane by chromatography on Sephacryl S...

Ornithine aminotransferase, a mitochondrial matrix enzyme, is expressed constitutively in many mammalian tissues and is subject to complex hormonal and dietary regulation in rat liver and kidney. The kidney enzyme is induced by estrogen and thyroid hormone (3,3',5-triiodo-L-thyronine, T3). We demonstrated previously that the hormonal induction in kidney is the result of an increase in ornithine...

conclusions the lee is a common finding among kidney transplant recipients. serial monitoring of aminotransferases, particularly alt, should be performed in all patients after kidney transplantation. patients and methods liver enzymes and other biochemical parameters were measured in all recipients. patients were divided into three groups, according to laboratory test time since transplantation...

We have constructeda computermodel,basedon experimentaldata and the known properties of the enzymes involved, of the aspartate aminotransferase and alanine aminotransferase assays by the coupled-enzyme procedure of Henry et al. [Amer. J. Clln. Pathol. 34, 381 (1960)] to assay sera from normal persons and persons with liver disease or myocardial infarct. Observed inhibitions of aminotransferase ...

Dexamethasone, insulin, and serum induce an additive increase in the activity of tyrosine aminotransferase (L-tyrosine : Z-oxoglutarate aminotransferase, EC 2.6.1.5) in hepatoma cells in tissue culture. By the criteria of immunotitration, heat stability, and electrophoresis on polyacrylamide gels, we have shown previously that all three effecters increase the cellular concentration of the same ...