I. An enzyme that catalyzes the reduction of erythrocyte cytochrome b 5 has been isolated from the supernatant fraction of erythrocyte hemolysates by chromatography on DEAE-cellulose, Amberlite CG-5 o, and Bio-Gel P-6o. 2. Erythrocyte cytochrome b 5 reductase has been shown to contain FAD. Incubation of the reductase in the absence of EDTA results in both the appearance of flavin fluorescence a...

A neurotoxin, designated toxin LY, has been isolated from the venom of the Egyptian cobra (Naja haje haje) by gradient chromatography on Amberlite CG-50, and has been further purified by gel filtration on Sephadex G-50. Homogeneity was verified by free boundary electrophoresis, acrylamide gel electrophoresis, sedimentation velocity, amino acid analysis, and end group analysis. Toxin LY has a se...

The bacteriolytic enzyme with an isoelectric point of 9.5 that is produced by all strains of Staphylococcus aureus investigated was purified from strain M18 (Wadström & Hisatsune, 1970). This enzyme released reducing groups from cell walls of Micrococcus lysodeikticus and was thus shown to be a bacteriolytic hexosaminidase. Although dinitrophenylation and acid hydrolysis of cell walls hydrolyse...

The determination of guanidoacetic acid in biological fluids has assumed greater importance since the demonstration that this compound is an intermediate in the biosynthesis of creatine (l-3). In the reported studies of creatine metabolism (4-6), guanidoacetie acid has been determined exclusively with the Sakaguchi reaction, which is given by monosubstituted guanidines. It has been shown recent...

Received April2, 1965 In 1960 Yamaoka et al. described one of the first well-documented abnormal hemoglobins to be found in Japan. This hemoglobin, designated Hb Shimonoseki for the city in which the family resides, constitutes approximately 20% of the hemoglobin of the heterozygotes and migrates with Hb S on paper electrophoresis at pH 8.6. The present communication concerns its further charac...

Crude glycerol (CG) is an abundantly available and cheap by-product from biodiesel production. Value-added applications for CG are highly wanted by industry and several processes such as the use of CG for enhanced oil recovery have been proposed. The aim of this study was to evaluate the sulfide production of sulfate-reducing bacteria (SRB) indigenous to oil reservoirs using CG as substrate. Th...

an oral suspension could a suitable dosage form for the geriatric patients. the calcium alginate coated ion exchange resinate of propranolol hydrochloride were prepared using amberlite ir-120 by solvent evaporation. microcapsules of propranolol hydrochloride resinate were prepared by solvent evaporation technique,the suspensions were prepared by using deionised water as the vehicle. methyl cell...

health care systems should assign quality improvement as their main mission. clinical governance (cg) is a key strategy to improve quality of health care services. the iranian ministry of health and medical education (mohme) has promoted cg as a framework for safeguarding quality and safety in all hospitals since 2009. the purpose of this study was to explore perceived facilitators and barriers...

Objective: Ion-exchangers are inert, water-insoluble polymers with ionizable functional groups on their surface. They can be used for purposes such as disintegrating or taste masking in orally dosage forms, they provide a pH-dependent controlled release.
 Material and Method: The loading release properties of different cation exchangers were investigated by Atenolol to Amberlite CG50, Dowe...