The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160) and to analyze the values according to the patient groups, infection localization and antimicrobial resistance. The following frequencies in all studied strains were established: algD (encoding GDP-mannose...

Mutations in the global regulatory genes gacS and gacA render Pseudomonas syringae pv. syringae strain B728a completely nonpathogenic in foliar infiltration assays on bean plants. It had been previously demonstrated that gac genes regulate alginate production in Pseudomonas species, while other published work indicated that alginate is involved in the pathogenic interaction of P. syringae on be...

Mucoid alginate-producing mutants of Pseudomonas aeruginosa are major pathogens in debilitating chronic pulmonary infections in patients with cystic fibrosis. The mucoid phenotype results from alginate biosynthesis whose genes are arranged in at least three chromosomal loci. Structural genes are located at the 34-min region and regulatory genes at 9 min. A third cluster at the 70 min region con...

Azotobacter vinelandii produces two polymers: the extracellular polysaccharide alginate and the intracellular polyester poly-beta-hydroxybutyrate (PHB). A cosmid clone (pSMU588) from an A. vinelandii gene library diminished alginate production by A. vinelandii mucoid strain ATCC 9046. The nucleotide sequence and predicted amino acid sequence of the locus responsible for the mucoidy suppression ...

Dual-species biofilms of Pseudomonas aeruginosa and Staphylococcus aureus generate difficultto-treat illnesses. Nutrition stress in affects physiology, microbial metabolism, species interactions, impacting bacteria growth survival. Furthermore, the function alginate, which is encoded by algD gene, production has been established. The present study aimed at investigating impact starvation on gen...