The dependence of the flux in the alcohol-degrading pathway on the activity of alcohol dehydrogenase was investigated in Drosophila larvae. Third-instar larvae were supplied with [2-13C]ethanol as a dietary carbon source. Specific carbon enrichments in de novo synthesized fatty acids were determined in vitro by means of 13C nuclear magnetic resonance spectroscopy. Carbon fluxes deduced from the...

It has been reported in literature that Z. mobilis has two types of alcohol dehydrogenase gene (ADH), one is Zinc inducible, and another is Iron (Ferrous) inducible. Therefore, it was attempted to increase the alcohol production by inducing the alcohol dehydrogenase activity by addition of Zinc ions. It was standardized that 10 mM Zinc sulphate addition to alcohol producing broth resulted in ma...

We examined the activities of class I and II alcohol dehydrogenase isoenzymes in the sera of patients with viral hepatitis using the fluorogenic substrates 4-methoxy-1-naphthaldehyde for class I and 6-methoxy-2-naphthaldehyde for class II. It was found that serum activities of class I and II alcohol dehydrogenase isoenzymes over the course of five weeks of hospitalisation were higher than those...

1. Yeast alcohol dehydrogenase was used to determine ethanol in the portal and hepatic veins and in the contents of the alimentary canal of rats given a diet free from ethanol. Measurable amounts of a substance behaving like ethanol were found. Its rate of interaction with yeast alcohol dehydrogenase and its volatility indicate that the substance measured was in fact ethanol. 2. The mean alcoho...

Thermoanaerobacter ethanolicus (ATCC 31550) has primary and secondary alcohol dehydrogenases. The two enzymes were purified to homogeneity as judged from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration. The apparent M(r)s of the primary and secondary alcohol dehydrogenases are 184,000 and 172,000, respectively. Both enzymes have high thermostability. They are tetram...

Using horse liver alcohol dehydrogenase, stereospecifically tritiated (R)and (S)-(y-3H)-coniferyl alcohol was synthesized. Using both of these substrates it was demonstrated that cinnamyl alcohol dehydrogenase from lignifying Forsythia tissue specifically removes the pro-R-hydrogen atom of coniferyl alcohol in the oxidation to the aldehyde. This also means that in the reverse reaction the A-hyd...

A pair of primers was designed to amplify the propylene alcohol dehydrogenase gene sequence based on the cDNA sequence of the tobacco allyl-alcohol dehydrogenase gene. All introns were sequenced using traditional polymerase chain reaction (PCR) methods and T-A cloning. The sequences from common tobacco (Nicotiana tabaccum L.) and rustica tobacco (Nicotiana rustica L.) were analysed between the ...

This study aimed to evaluate the antioxidant and hepatoprotective effects of aqueous 60% ethanol extracts Allium ochotense Prokh. against alcohol-induced cytotoxicity as well on activities alcohol-metabolic enzymes. Antioxidant were analyzed using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl, ferric reducing power, malondialdehyde assays, found that both exhibited consid...

We read with a great pleasure the article ‘Rare ADH variant constellations are specific for alcohol dependence’ by Zuo et al. (2012). We want to direct the speciality about the alcohol dehydrogenase (ADH) isoenzyme nomenclature to the readers of Alcohol and Alcoholism. Namely, the authors use an old isoenzyme nomenclature what can cause some confusion to not enough familiar readers. Electrophor...