The relationship between Ca++ and pinocytosis was investigated in Amoeba proteus. Pinocytosis was induced with 0.01% alcian blue, a large molecular weight dye which binds irreversibly to the cell surface. The time-course and intensity of pinocytosis was monitored by following the uptake of [3H]SUCROSE. When the cells are exposed to 0.01% alcian blue, there is an immediate uptake of sucrose. The...

entire lesion was fixed in Bouin's fluid. The specimen was sectioned and stained with hematoxylin and eosin, Bodian's silver impregnation, phosphotungstic acid-hematoxylin, Masson's trichrome, PAS2 -Alcian blue, toluidine blue at pH 2 and pH 4, and Gomori's reticulum stain. This lesion is registered as Specimen RTLA 272 in the Registry of Tumors in Lower Animals, Museum of Natural History, Smit...

were CEA negative and Leu-M1 or B72.3 positive have been reported. These latter two antibodies were not used by the authors. Furthermore, ultrastructural studies have been found useful by a number of authors,22 6 but were not employed in the study by Beuachamp et al,t Some ofthese tests may not have been performed due to the limitation of the sample size. This merely serves to underscore the di...

Transparent exopolymer particles (TEP) are ubiquitous in marine and freshwaters, and have been subject to intensive study by oceanographers and limnologists over the past 15 years. These microscopic organic particles (visualized by Alcian Blue staining for acid polysaccharides) may be considered a planktonic form of exopolymeric substances (EPS). Two aspects relating to the potential involvemen...

Various imaging techniques have been widely used to study intervertebral disc (IVD) biology, among which histological methods are most common as they are indispensable tools to retrieve structural information. Stainings using Alcian blue-fast red and safranin-O-fast green are popular but they have limitations in delineating the complex structures of the IVD comparing to their use for articular ...

1. Weanling rats, aged 21 d, given a 40 g casein/kg diet for 25 d were compared with ‘age-matched’ controls given a 200 g casein/kg diet and with ‘weight-matched’ weanling controls. 2. The protein-malnourished rats demonstrated failure of weight gain and linear growth, hypoproteinaemia, hypoalbuminaemia and fatty liver. 3. Autoradiography of the tibia was carried out 24 h after intraperitoneal ...