The study was designed for isolation and characterization of azo dye decolourizing bacteria which is a prerequisite for developing a microorganism-facilitated treatment of polluting dyes. In this study nine types of bacteria which were able to decolourize three types of azo dyes (Blue H/C, Red 3B and Yellow 3R dye) were isolated from textile effluents collected from Gazipur industrial area in B...

A lysozyme-osmotic shock method is described for fractionation of Alcaligenes faecalis which uses glucose to adjust osmotic strength and multiple osmotic shocks. During phenylethylamine-dependent growth, aromatic amine dehydrogenase, azurin, and a single cytochrome c were localized in the periplasm. Their induction patterns are different from those for the related quinoprotein methylamine dehyd...

Achromobacter piechaudii strain HLE is a betaproteobacterium (previously known as Alcaligenes faecalis) that was an early isolate with arsenite oxidase activity. This draft genome of 6.89 Mb is the second available genome for this species in the opportunistic pathogen Alcaligenaceae family.

Conn (1942) observed that Alcaligenes faecalis required organic nitrogen, but as observed by Porter (1946) no report was found of a study of nitrogen compounds suitable for continued growth of A. faecalis. It was reported, however, that this organism grew in a medium containing NH4 lactate as a source of nitrogen and carbon (Braun and Cahn-Bronner, 1921), and used NH4NO3, asparagine, cysteine, ...

A phenanthrene-degrading bacterium that assimilated a wide range of organic compounds was isolated from a soil sample and identified as Alcaligenes faecalis strain AFK2. The strain degraded phenanthrene through protocatechuate, but did not utilize naphthalene. The phenanthrene-degrading phenotype (Phn+) of AFK2 disappeared after 20 successive subcultures in a mineral salts medium containing o-p...

Alcaligenes faecalis No.4, which is capable of heterotrophic nitrification and aerobic denitrification was used to treat coking wastewater (CW) to remove high-strength of ammonium and phenol. First, the phenol-degrading ability of No.4 was verified and a synthetic medium containing lactate and phenol as carbon sources was subsequently applied to the No.4 culture. Both carbon materials were enti...

Exo-polysaccharides (EPS) are environment friendly natural polymers secreted by microorganisms in the surrounding medium. Due to the presence of unique structural composition, EPS shows diverse applications such as in food formulations, pharmaceutical, and cement based construction industry, etc. In the present investigation, the bacteria producing higher exopolysaccharide was screened among ba...

A polyphasic taxonomic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analysis, whole-cell protein and fatty acid analyses, AFLP (amplified fragment length polymorphism) fingerprinting and an extensive biochemical characterization was performed on 10 strains provisionally identified as Alcaligenes faecalis-like bacteria. The six environmental and fo...

Four nitrite-dissimilating strains, isolated from Weser Estuary sediments, were investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the 'Betaproteobacteria' and are related to the genus Alcaligenes. The highest level of sequence similarity (100 %) was found with strain M3A (=ATCC 700596), a dimethyl su...

To analyze the status of the genus Alcaligenes in the agricultural environment, we developed a PCR method for detection of these species from vegetables and farming soil. The selected PCR primers amplified a 107-bp fragment of the 16S rRNA gene in a specific PCR assay with a detection limit of 1.06 pg of pure culture DNA, corresponding to DNA extracted from approximately 23 cells of Alcaligenes...