Maik Münzinger, Kambiz Taraz and Herbert Budzikiewicz* Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Köln, Germany. Fax: + 4 9 -2 21 -470 -5057 . E-mail: [email protected] * Author for correspondence and reprint requests Z. Naturforsch. 54c, 867-875 (1999); received July 8, 1999 Ralstonia eutropha, Iron Transport, Siderophore, Staphyloferrin B The struct...

The flavohemoprotein (FHP) encoding gene of the strictly respiratory Gram-negative bacterium Alcaligenes eutrophus was isolated from a megaplasmid library by using FHP-specific antibodies and oligonucleotide probes based on the amino-terminal polypeptide sequence of FHP, determined previously (Zhu, H., and Riggs, A. F. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5015-5019). The fhp gene codes for ...

Mutants defective in chemolithoautotrophic growth (Aut-) have been isolated from Alcaligenes eutrophus strains H16, N9A, G27, and TF93. Spontaneous Aut- mutants were obtained only with strain TF93. Mutants of the other strains were selected after conventional mutagenesis or treatment with mitomycin. Most of the mutants, including the spontaneous Aut- strains, lacked hydrogenase activity (Hox-) ...