RNA interference (RNAi) is a powerful reverse genetic tool to study gene function. The data presented here show that Agrobacterium rhizogenes-mediated RNAi is a fast and effective tool to study genes involved in root biology. The Arabidopsis gene KOJAK, involved in root hair development, was efficiently knocked down. A. rhizogenes-mediated root transformation is a fast method to generate advent...

Background The wide adaptability, fast growth rate, good form, excellent wood and fiber properties make Eucalyptus the most widely planted genus of plantation forest trees. With the availability of Eucalyptus genome sequence information, assignment of function to genes contributing to desired traits require functional validation via overexpression or silencing in the context of the plant enviro...

Agrobacterium tumefaciens and A. rhizogenes transport single-stranded DNA (ssDNA; T-strands) and virulence proteins into plant cells through a type IV secretion system. DNA transfer initiates when VirD2 nicks border sequences in the tumour-inducing plasmid, attaches to the 5' end, and pilots T-strands into plant cells. Agrobacterium tumefaciens translocates ssDNA-binding protein VirE2 into plan...

CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/Cas9 to precisely knock out the committed diterpene synthase gene (SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a traditional Chinese medicinal herb with significant pharmacologica...

Crown gall disease caused by Agrobacterium tumefaciens is considered as the main bacterial disease of stone fruit rootstocks in Tunisia against which only prophylactic control measures are available. Therefore, twelve rhizobacterial strains and two reference antagonists Agrobacterium rhizogenes K84 and K1026 were investigated for their efficacy against the causal agent of the disease in vitro, ...

Catharanthus roseus L. is a medicinal plant that produces numerous indole terpenoid alkaloids, including vincristine and vinblastine, which are used for cancer treatment. The effect of specified precursors (L-phenylalanine, L-tyrosine) elicitors (chitosan, methyl jasmonate) on C. hairy roots (CHR) growth has been examined in order to increase the content vinblastine. Our results showed CHR gene...

Limonium bicolor is a perennial herbaceous plant belonging to the Plumbaginaceae family. It can be used as dried flower or in cut arrangements and serves model recretohalophyte. Its genome sequencing has been recently completed. However, research on L. limited by absence of highly efficient genetic transformation system. In this study, we established Agrobacterium rhizogenes-mediated method. Th...