Anatomical analysis of the plant tissues is an important technique used in research for studying process growth, development, adaptation to environmental stress as well characterizing gene localization. In Vietnam, most anatomical material was manually conducted which time-consuming and labor-intensive. this report, we present innovative method that can deliver high-quality rice root sectioning...

Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous bioactive hydrogels. Polysaccharides ...

BACKGROUND The electrical properties of agarose gel, namely impedance and capacitance, are relatively unexplored. Agarose gels are used as in vitro models in studies across numerous disciplines, including imaging, radiotherapy, infusion, and neurosurgery. PURPOSE In this study, we seek to characterize the impedance response of low concentration agarose gels by relating the gel concentrations ...

In order to improve the gelling properties of agarose, we modified it by methylation. The agarose was prepared from Gracilaria asiatica, G. bailinae, and G. lemaneiformis with alkaline, treated with diatomaceous earth and activated carbon, and anhydrous alcohol precipitation. The methylation reaction process of agarose was performed with dimethyl sulfate while the chemical structure of low-gell...

In the last decade the Drosophila larva has evolved into a simple model organism offering the opportunity to integrate molecular genetics with systems neuroscience. This led to a detailed understanding of the neuronal networks for a number of sensory functions and behaviors including olfaction, vision, gustation and learning and memory. Typically, behavioral assays in use exploit simple Petri d...

Suppression reagent according to the instruction manual and then subjected to sequence analysis using an ABI PRISM310 Genetic Analyzer. In an effort to establish optimal conditions for the direct sequencing of plasmid DNA within bacterial colonies or λDNA within single-phage plaques, a semi-quantitative investigation of the inhibitory effects of LB medium and agarose gel was carried out. It was...

Using rat 13762NF mammary tumor cell clones of varying spontaneous metastatic potentials and biochemical properties and known phenotypic stabilities we studied the relationship between cell colony growth in a clonogenic assay and the biological and biochemical properties of cells derived from different cell colonies. The spontaneous metastatic potential of in vivo or In vitro grown 13762NF tumo...

The objective of this work was to select the most suitable procedure to immobilize lipase from Penicillium camembertii (Lipase G). Different techniques and supports were evaluated, including physical adsorption on hydrophobic supports octyl-agarose, poly(hydroxybutyrate) and Amberlite resin XAD-4; ionic adsorption on the anionic exchange resin MANAE-agarose and covalent attachment on glyoxyl-ag...

Under-agarose chemotaxis has been used previously to assess the ability of neutrophils to respond to gradients of chemoattractant. We have adapted this assay to the chemotactic movement of Dictyostelium amoebae in response to folic acid. Troughs are used instead of wells to increase the area along which the cells can be visualized and to create a uniform front of moving cells. Imaging the trans...