In the Replireader system for identifying Enterobacteriaceae, plates of biochemical media are inoculated with a replicator and the results are put into a computer. The machine correctly identified 92x2% of 734 strains of Gram-negative bacilli isolated from urine; it was incorrect in 0x8% and failed to recognise 7%. The Replireader was also used to record the results of sensitivity tests using a...

The in vitro susceptibility of 20 isolates of Clostridium difficile to cefotaxime, moxalactam, and cefoperazone was determined by a standard agar dilution method. The median minimal inhibitory concentrations were 64, 32, and 32 mug/ml for cefotaxime, moxalactam, and cefoperazone, respectively.

MICs of clarithromycin and amoxycillin for 253 isolates of Helicobacter pylori were measured by an air-dried microplate method and compared with the results obtained by the agar plate dilution method. The air-dried microplate method is performed by coating each well of a 96-well microplate with the test antibiotic and air-drying it. There were no marked differences between the air-dried micropl...

The effects of inocula and media on the activities of ampicillin, penicillin, chloramphenicol and co-trimoxazole against Haemophilus influenzae were examined in vitro. Two inocula and four media were tested by the disk diffusion, broth dilution, and agar dilution methods. Chloramphenicol activity versus H. influenzae was least affected by changes in inocula and media, whereas co-trimoxazole was...

AIMS To investigate the evolution of chromosomal and plasmid mediated resistance for ampicillin and tetracycline of N gonorrhoeae strains in Stockholm during 1982-1993. METHODS A total of 404 gonococcal strains isolated in 1982, 1987, 1990, 1992, 1993 were analysed for minimal inhibitory concentrations (MIC) of ampicillin and tetracycline and for plasmid content. MIC values were determined by...

The increasing prevalence of penicillin-resistant Streptococuus pneumoniae urges for fast and accurate susceptibility testing methods. This study evaluated the comparability of three commonly used techniques; disk diffusion, E-test and agar dilution, to detect penicillin susceptibility in clinical isolates of S. pneumoniae. Fifty pneumococcal isolates, obtained from patients at the University o...

Neisseria meningitidis is one of the important cause of meningitis and has been extremely susceptible to penicillin. Nevertheless, moderately penicillin resistant strains have been reported in some parts of the world. To our knowledge, there is no such report in Malaysia. We report two clinical isolates that were found to have MICs of decreased susceptibility to penicillin by the agar dilution ...

Recent studies have questioned treatment of serious methicillinresistant Staphylococcus aureus (MRSA) infections with vancomycin because of treatment failures, despite vancomycin MICs in the susceptible category (MIC 2 g/ml) (2, 3, 5, 6, 7). Some of these studies have used broth microdilution for determining vancomycin MICs (5), while others have used the Etest commercial product (AB Biodisk, S...

MICs of amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, and imipenem were determined by Etest for 100 clinical strains of rapidly growing mycobacteria and compared with MICs determined by a reference agar dilution method. Etest MICs were also determined by an alternative inoculum application (agar overlay) method and compared with MICs determined by the inoculum application met...