Background: Differentiation, migratory properties and availability of Mesenchymal Stromal Cells (MSC) have become an important part of biomedical research. However, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells. Objective: To characterize and compare the phenotype and cytokines of adipose derived MSCs (...

IntroductionOver the past decade, human adipose-derived mesenchymal stem/signaling/stromal cell (ADSC) therapies have become increasingly popular as a biologic treatment for variety of orthopedic conditions. ADSCs represent source stromal cells with properties that allow paracrine and immunomodulatory functions ability to differentiate into various downstream cellular tissues. Before considerin...

The aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. Rat large intestine was dissected in fragments and decellularized by physicochemical methods. The scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. Microscopic sections were prepared fr...

objective(s) some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. in the present study proliferation capacity and aging of such cells were explored. materials and methods adherent cells were isolated from the collagenase digests of adipose tiss...

Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. Stemness and multipotential features of Mesenchymal Stem Cells (MSC) has been highlighted in many studies but there are many dark aspects in ex...

introduction: mesenchymal stem cells (mscs) hold great promise in the field of regenerative medicine.although originally isolated from bone marrow, mscs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. stemness and multipotential features of mesenchymal stem cells (msc) has been highlighted in many studies but there are many dark aspects in exc...

Various in vitro preconditioning strategies have been implemented to increase the regenerative capacity of MSCs. Among them are modulation culture atmosphere (hypoxia or anoxia), three-dimensional (3D), addition trophic factors (in form growth factors, cytokines hormones), lipopolysaccharides, and pharmacological agents. Preconditioning mesenchymal stem cells by culturing a hypoxic environment,...