Adenylate kinase and downstream AMP signaling is an integrated metabolic monitoring system which reads the cellular energy state in order to tune and report signals to metabolic sensors. A network of adenylate kinase isoforms (AK1-AK7) are distributed throughout intracellular compartments, interstitial space and body fluids to regulate energetic and metabolic signaling circuits, securing effici...

Amino acid analysis, HPLC separation of trypsin digests, and sequence analysis showed that the thermosensitivity of the adenylate kinase (EC 2.7.4.3) from Escherichia coli K-12 strain CR341 T28 results from substitution of a serine residue for proline-87 in the wild-type enzyme. This mutation is accompanied by decreased affinity for nucleotide substrates and decreased catalysis. Circular dichro...

Adenylate kinase (AK) catalyse the phosphotransferase reaction plays an important role in cellular energy homeostasis. The inhibitors of bacterial AK are useful in the treatment of several bacterial infections. To the novel inhibitors of AK, docking studies performed by using the 3D structure of Bacillus stearothermophilus adenylate kinase from protein data bank (IZIP). 46 Quinoxaline analogues...

The phospholipase C-mediated hydrolysis of phosphatidylcholine has been shown recently to be activated by a number of agonists. Muscarinic receptors, which trigger various signal transduction mechanisms including inhibition of adenylate cyclase through Gi, have been shown to be potent stimulants of this novel phospholipid degradative pathway. We demonstrate here, by exogenous addition of Bacill...

Proton relaxation rate (PRR) studies indicated that there was little direct interaction between manganous ion and adenylate kinase. However, a large enhancement of the PRR of water was observed in the presence of ATP; for the enzyme combined with manganous-ATP complex rather than directly with the metal ion. Thus adenylate kinase is similar to creatine kinase but not to pyruvate kinase, with re...

In vitro cell cultures are used as models for drug discovery. The detection of damage biomarkers such adenylate kinase (AK) is often in screening and biology experiments. A microfluidic platform AK was developed with the capability detecting resulting from lysis 10–100 human colorectal adenocarcinoma HCT116 cells. For this assay, captured on surface microbeads integrated into a device optically...

Energy charge responses of yeast hexokinase, rabbit muscle pyruvate kinase, soluble rat brain hexokinase, and rabbit muscle phosphofructokinase were observed. Several additional factors that were shown to influence markedly the sensitivity of these enzyme responses to the adenylate energy charge include pH, total adenylate concentration, levels of non-adenylate reaction products, and non-adenin...

Following acute stroke, creatine kinase and other enzymes are released into the cerebrospinal fluid and blood from injured brain tissue. To determine whether regional differences in brain enzyme activity might exist and therefore affect the amount of enzyme released, we quantified the levels of creatine kinase, adenylate kinase, and lactate dehydrogenase in 12 regions of normal canine brain (n ...