Moat plots of enzyme concentration versus catalytic activity are linear, or fall on a single arm of an hyperbola, allowing treatment in the double reciprocal manner of "maximum rate of product formation" kinetics. Since curves of similar, if undefined, shape arise from many experimental measurements, the double reciprocal approach with its characterizing constants, can be employed to give an ap...

In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time ...

Free radicals are unstable molecules caused by several electrons in the molecule that does not have a partner. can potentially trigger onset of various degenerative diseases. One efforts be made to ward off disease is consuming antioxidant compounds. Antioxidant compounds found plants with genus Allium, such as Allium cepa L. This study aims determine activity, flavonoid concentration, and phen...

Background and objectives: Resistance towards reveling antibiotics has captured great interest in evaluating the antimicrobial properties of the natural plants. Taraxacum mongolicum is widely used as a folklore medicinal plant for its diuretic, antirheumatic and anti-inflammatory properties. Though there are some reports on antimicrobial properties of Taraxacum mongolicum, stu...