Abstract Background Actin is an essential cellular protein that assembles into microfilaments and regulates numerous processes such as cell migration, maintenance of shape, material transport. Methods In this study, we explored the effect actin polymerization state on osteogenic differentiation human adipose-derived stem cells (hASCs). The hASCs were treated for 7 days with different concentrat...

We examined the actin-nucleating activity in polymorphonuclear leukocyte lysates prepared at various times after chemotactic peptide addition. The actin nucleation increases two- to threefold within 15 s after peptide addition, decays to basal levels within 90 s, and is largely independent of cytoplasmic calcium fluxes. The peptide-induced nucleation sites behave as free barbed ends and therefo...

The stratified skin epidermis needs dynamic cell rearrangement to self-renew while maintaining tissue integrity guarantee barrier function in the face of continuous mechanical stress. contractile actomyosin cytoskeleton connects with intercellular adheres junctions enable cellular rearrangements and migration, whereas physical properties keratin intermediate filament through its interaction des...

Follicular dendritic cells (FDCs) are stromal that reside in follicles of secondary and tertiary lymphoid organs germinal centres. They provide structure to the tissue micro-environment promote proliferation, activation, affinity maturation B cells. FDCs express high levels complement receptor 1/2 (CD21/35) on their surface retain immune complexes for presentation It has been suggested FDC acti...

Abstract The actin is one of the main component eukaryotic cytoskeleton. continuous rearrangement filaments provided by different complexes with divalent cations (Ca 2+ or Mg ) and nucleotides (ATP, ADP). In medical routine, cyclophosphamide (CP) applied as cytostatic it was shown that in vivo muscle filament system changed CP treatment has direct interaction monomers well. evolutionary importa...

Physarum profilin reduces the rates of nucleation and elongation of F-actin and also reduces the extent of polymerization of actin at the steady state in a concentration-dependent fashion. The apparent critical concentration for polymerization of actin is increased by the addition of profilin. These results can be explained by the idea that Physarum profilin forms a 1:1 complex with G-actin and...

Filamin A (FLNa) can effect orthogonal branching of F-actin and bind many cellular constituents. FLNa dimeric subunits have N-terminal spectrin family F-actin binding domains (ABDs) and an elongated flexible segment of 24 immunoglobulin (Ig) repeats. We generated a library of FLNa fragments to examine their F-actin binding to define the structural properties of FLNa that enable its various func...