نتایج جستجو برای: acid fast staining
تعداد نتایج: 1025504 فیلتر نتایج به سال:
The principles and techniques involved in fluorescence microscopy as related to the demonstration of acid-fast bacilli are discussed. The auramine-rhodamine staining method, for both tissue and bacteriological preparations, is described as well as the necessary equipment for both microscopy and photomicroscopy. Fluorescence staining is no more difficult nor time consuming than a Ziehl-Neelsen s...
OBJECTIVES To evaluate the routine performance and the technical parameters of different acid-fast staining methods: Kinyoun, Ziehl-Neelsen (ZN), auramine, and auramine-rhodamine. DESIGN AND PARTICIPANTS The performance of 167 laboratories was analyzed using prestained and unstained slides. SETTING Laboratories holding New York State permits. RESULTS The results revealed that Kinyoun's co...
Introduction: Cryptosporidiosis is prevalent world wide, causing a variety of problems ranging from acute, self-limiting diarrhea to fatal cases in immunocompromised persons, particulary those with acquired immunodeficiency (AIDS). Diagnosis of Cryptosporidium is made by identification of oocysts in stool specimens. The detection is most commonly made by the acid-fast staining method followed b...
Background: Cryptosporidiosis is prevalent in animals and human beings, both immuno-competent and immuno-compromised persons, worldwide. Epidemiologically, it is important to differentiate between human and animal species. The aim of this study is to define the utility of a 1055 bp fragment of 18s rRNA for differentiation of Human and Cattle Cryptosporidiosis. Material and methods: 1020 human...
The present study was carried out for the detection and identification of bovine tuberculosis (bTB) using polymerase chain reaction (PCR). A total of 10 suspected cattle of Savar and BAU dairy farm were examined. Lymphnode biopsy, nasal swabs and blood were collected. Smears from lymphnode biopsy and nasal swabs were made onto clear slides and stain with acid fast staining. Portion of lymohnode...
A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In additio...
Acid fast bacteria (AFB) are cell walls containing complex lipids so that in Ziehl-Neelsen staining they can bind dyes because not washed off by 3% HCl Alcohol (Alcoholic Acid). One of the host factors pulmonary tuberculosis is smoking habits and exposure. Smoking will damage defense mechanism lungs, which causes lungs to be more easily infected with germs. The purpose this study was determine ...
Objective] Fluorescent staining is of paramount importance, not only for confi rming the presence of mycobacteria in a given specimen but also for providing an estimated growth quantifi cation. In this study, for rapidly growing Mycobacterium fortuitum, we evaluated the effectiveness of a rapid fl uorescent staining method employing auraminerhodamine (AR) fl uorescent stain and acridine-orange ...
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