Inclusion bodies produced in Escherichia coli are composed of densely packed denatured protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive forms is cumbersome, results in poor recovery and accounts for the major cost in production of recombinant proteins from E. coli. With new information available on the structure and function of protein aggregates i...

Lignin is an abundant and renewable source capable of replacing different raw materials in the chemical industry. It can be obtained from lignocellulosic biomass (LCB) via pretreatment methods. In present study, hydrolysis lignin (HL) SunburstTM technology was utilized to investigate its enzymatic conversion. At first, soluble HL fractions were alkali solubilization followed by acid precipitati...

introduction: the aim of this study was to evaluate the compressive strength of calcium-enriched mixture (cem) cement in contact with acidic, neutral and alkaline ph values. methods and materials: the cement was mixed according to the manufacturer’s instructions, it was then condensed into fourteen split molds with five 4×6 mm holes. the specimens were randomly divided into 7 groups ( n =10) an...

The present study deals with the effect of increased concentrations of alcohols (methanol and ethanol) on the solubilization of FePO4 and CaHPO4 by Aspergillus niger. The efficiency of solubilization (ES) of inorganic phosphates, fungal growth, acid production and pH variation were determined. The concentrations of alcohols that most favored the solubilization of phosphates were 4% methanol and...

Hair is composed of proteins, lipids, water, and small amounts of trace elements. All proteins in animal and human bodies are built from permutations of amino acid molecules in a polypeptide string. The polypeptide chains of protein keratin are organized into filaments in hair cells. Hair is one of the most difficult proteins to digest or solubilize. Among the most common dissolving procedures ...

Bile salts are essential for phospholipid secretion into the bile. To study the relevance of the structure of phospholipids for their interaction with bile salts, we used spin-labeled or fluorescent phospholipid analogues of different head groups and acyl chain length. Those analogues form micelles in aqueous suspension. Their solubilization by bile salts resulting in the formation of mixed mic...

Burkholderia cepacia CC-Al74 with a high ability for solubilizing tricalcium phosphate (TCP) was used to study the P-solubilization mechanism. We collected filtrates able to solubilize TCP from the cultures of strain CC-Al74 and demonstrated that the P-solubilization increased from 0 microg ml(-1) to 200 microg ml(-1) during exponential growth, when the pH decreased from 8 to 3. HPLC-analysis r...

chemical injuries of the cornea are ophthalmic emergencies. these injuries often result in significant ocular morbidity and generally strike young adults in the prime years of life. classification schemes enable the ophthalmologist to determine the severity of injury and the prognosis for the injured eye. immediate management consists of copious irrigation following exposure. effective manageme...