Ribosome-inactivating proteins (RIPs) are enzymes (N-glycosidases classified as rRNA N-glycohydrolases, EC 3.2.2.22), mostly of plant origin, but also bacterial, fungal and algal which cause irreversible inhibition protein biosynthesis carried out by ribosomes superior organisms some bacteria. The enzymatic activity consists specific depurination that renders them unable to interact with elonga...

In this study we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of abrin by using catalytic AuNPs for the first time. AuNPs possess the peroxidase-like activity that can catalyse 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, leading to color change of the solution. It is interesting to find that the peroxidase-like activity of AuNPs can be imp...

For the convenience of fast measurement in the outdoor environment, a portable electrochemiluminescence biosensor with the screen-printed electrode as the reaction center was developed, which possesses the characteristics of high sensitivity, small scale, simplified operation and so on, and has been used for in situ detection of abrin. First, combining with magnetic separation technique, the "b...

BACKGROUND When discussing known poisons and their clinical consequences, few physicians are aware of the deadly poison abrin. The common symptoms of abrin toxicity include nausea, vomiting, abdominal pain, and diarrhea with or without bloody stool. However, with an estimated fatal human dose of less than 1 microgram/kg, death due to complications such as liver failure, renal failure, and cereb...

Modeccin was extracted from the root of Adenia digitata and purified by gel filtration on Sephadex G-100, ion exchange chromatography on DEAE-cellulose, and affinity chromatography on a column containing immobilized, neuraminidase-treated fetuin. The toxin was eluted from the affinity column with lactose. Polyacrylamide gel electrophoresis of affinity-purified modeccin in the presence of sodium...

The effects of retinoic acid and 12-O-tetradecanoylphorbol 13-acetate on the sensitivities of a number of cell lines to the toxins modeccin, abrin, ricin and diphtheria toxin were studied. Retinoic acid and some other retinoids were found to protect a number of the cell lines against the toxins. HeLa cells that were protected bound much more retinoic acid than L-cells that were not protected. T...

The advent of cell fusion techniques for producing monoclonal antibodies has stimulated world-wide effort in the search for antibodies with specificity for neoplastic cells. In parallel, a number of laboratories have attempted to devise ways of attaching cytotoxic agents to antibodies with the aim of generating potent anticancer agents from monoclonal antibodies that exhibit sufficient specific...

The antitumor effect of combinations of the toxic proteins ricin and abrin with other drugs was studied in mice with systemic L1210 leukemia. Ricin was tested in combination with daunorubicin, c/s-dichlorodiammineplatinum(ll), or vincristine, and abrin was tested in combination with Adriamycin. The antileukemic effect of the regimens was gauged by assaying the life span of tumor-bearing animals...