Background: Hepatocytes transplantation (HCTx) is an alternative method to orthotopic liver transplantation used in treatment of several liver injuries. It can be used either fresh or as encapsulated hepatocytes. Expression of Hep Par 1, CK7 and Albumin were performed to identify and analyze the transplanted isolated adult hepatocytes in hamsters spleens. Aim: This work aimed to study the expre...

Disturbed proteostasis as a particular phenotype of the aging organism has been advanced in C. elegans experiments and is also conceived to underlie neurodegenerative diseases in humans. Here, we investigated whether particular changes in non-disease related proteostasis can be identified in the aged mammalian brain, and whether a particular signature of aberrant proteostasis is related to beha...

Background One of the hallmarks Alzheimer’s disease (AD), an age-related progressive form dementia, is accumulation amyloid beta (Aβ42) plaque, which manifests in decline cognitive function. The these Aβ42 plaques trigger hyperphosphorylation tau, a microtubule associated protein, results intracellular neurofibrillary tangles (NFTs) due to destabilization microtubules. Method We employed Gal4/U...

Aberrant promoter DNA-hypermethylation and repressive chromatin constitutes a frequent mechanism of gene inactivation in cancer. There is great interest in dissecting the mechanisms underlying this abnormal silencing. Studies have shown changes in the nuclear organization of chromatin in tumor cells as well as the association of aberrant methylation with long-range silencing of neighboring gene...

Hypermethylation of CpG islands is a common mechanism by which tumor suppressor genes are inactivated. We studied 45 pancreatic carcinomas and 14 normal pancreata for aberrant DNA methylation of CpG islands of multiple genes and clones using methylation-specific PCR (MSP) and bisulfite-modified sequencing. Using MSP, we detected aberrant methylation of at least one locus in 60% of carcinomas. T...

Chlorophylls (Chl) in photosynthetic apparatuses, along with other macromolecules in chloroplasts, are known to undergo degradation during leaf senescence. Several enzymes involved in Chl degradation, by which detoxification of Chl is safely implemented, have been identified. Chl degradation also occurs during embryogenesis and seedling development. Some genes encoding Chl degradation enzymes s...

The frequency distribution of mutation-induced aberrant 3' splice sites (3'ss) in exons and introns is more complex than for 5' splice sites, largely owing to sequence constraints upstream of intron/exon boundaries. As a result, prediction of their localization remains a challenging task. Here, nucleotide sequences of previously reported 218 aberrant 3'ss activated by disease-causing mutations ...

The frequency distribution of mutation-induced aberrant 30 splice sites (30ss) in exons and introns is more complex than for 50 splice sites, largely owing to sequence constraints upstream of intron/ exon boundaries. As a result, prediction of their localization remains a challenging task. Here, nucleotide sequences of previously reported 218 aberrant 30ss activated by disease-causing mutations...

Most human tumors are characterized by: (1) an aberrant set of chromosomes, a state termed aneuploidy; (2) an aberrant gene expression pattern; and (3) an aberrant phenotype of uncontrolled growth. One of the goals of cancer research is to establish causative relationships between these three important characteristics. In this paper we were searching for evidence that aneuploidy is a major caus...