A botanical extract (Regrapex-R) prepared from whole grape (Vitis vinifera) and Polygonum cuspidatum, which contains polyphenols, including flavans, anthocyanins, emodin, and resveratrol, exhibited dose-dependent scavenging effects on reactive oxygen species (ROS). The extract inhibited increases of ROS and protein carbonyl in isolated rat liver mitochondria following exposure to 2,2'-azobis (2...

Creatine (Cr) is naturally produced in the body and stored in muscles where it is involved in energy generation. It is widely used, especially by athletes, as a staple supplement for improving physical performance. Recent reports have shown that Cr displays antioxidant activity which could explain its beneficial cellular effects. We have evaluated the ability of Cr to protect human erythrocytes...

4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellata root extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and α-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,2'-az...

Introduction: This study was designed to compare the plasma lipid peroxidation (LPO) levels in preterm and full-term neonates and their respective mothers, to assess their relationship with the degree of oxidative stress and the levels of vitamin C, an important antioxidant of the body. Material and methods: The studied groups included 70 neonates, 30 preterm (24-36 weeks of gestation) and 40 f...

Protein instability is a major obstacle in the production and delivery of monoclonal antibody-based therapies for cancer. This study presents real-time isothermal differential scanning fluorimetry as an emerging method to evaluate the stability of human immunoglobulin G protein with high sensitivity. The stability of polyclonal human immunoglobulin G against urea-induced denaturation was assess...

Vernonia cinerea (VC) Less has been proposed as a medicinal plant with interesting activities, such as an aid for smoking cessation worldwide. Despite its previous clinical success in smoking cessation by exhibiting reduced oxidative stress, it has not been approved. The aim of this study was to investigate various antioxidant activity and active compounds that have not been approved, including...

We have demonstrated a novel antioxidant action of ethanolamine plasmalogens both in protecting cholesterol from oxidation by free radicals and in lowering the oxidizability of membranes, along with the action of scavenging radicals, by the oxygen-uptake method using large unilamellar vesicles and the water-soluble azo-radical initiator, AAPH [2,2'-azobis-(2-amidino-propane) dihydrochloride].

Thyme species produce a wide variety of phenolic compounds including tannins, acids, and flavonoids. Thymus atlanticus (T. atlanticus) willdenowii willdenowii) are important thyme in the southeast Morocco, with numerous biological properties. The polyphenolic extracts these two were obtained using ethanol through Soxhlet apparatus. Antioxidant (DPPH, FRAP, TAC methods), antihemolytic (2,2'-Azob...

In this study, the interaction between clozapine, an atypical antipsychotic drug, and alpha-2-macroglobulin (?2M), a multipurpose anti-proteinase, was investigated under simulated (patho) physiological conditions using multiple spectroscopic techniques molecular modeling. It found that ?2M binds clozapine with moderate affinity (the binding constant of 0.9 × 105 M?1 at 37 °C). The preferable si...