An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...

Almond oil is used in many cosmetic products and as a snack in confectionery (marzipan, "turrón", nougat), food products (almond milk, ice cream, chocolate), culinary recipes and also cosmetic base. Seeds of 18 almond genotypes and cultivars: Azar, Rabie, A200, Shahroud13, Zodgol2, A230, Tuono, Shahrekord1, Shahroud 21, Najafabad, Nonpareil, Zodgol1, Yalda, K12-8, Supernova, Shekofeh, Sahand...

Reverse transcription followed by quantitative polymerase chain reaction analysis, or qRT-PCR, is an extremely sensitive, cost-effective method for quantifying gene transcripts from plant cells. The availability of nonspecific double-stranded DNA (dsDNA) binding fluorophors, such as SYBR Green, and 384-well-plate real-time PCR machines that can measure fluorescence at the end of each PCR cycle ...

For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate University. The RNA isolated using this standardized protocol was high in quality and led to successful good ...

Premedication should provide effective anxiolysis and conscious sedation to improve the conditions for parental separation and induction of general anesthesia. Among different premedicants used for children by the oral route, we used in this study midazolam and fentanyl aiming to determine the value of adding fentanyl lozenge (brand name Actiq®, Cephalon, UT) to midazolam syrup for preoperative...