In this study, the binding of amlodipine (a Ca ++ channel Blocker) and arsenic (metalloid) to bovine serum albumin (BSA) was studied by equilibrium dialysis(ED) method in order to have an insight into their binding chemistry to BSA. Free amlodipine concentration was increased due to addition of arsenic which reduced the binding of the compounds to BSA. However, the free fraction was not increa...

PURPOSE High-mobility group box 1 protein (HMGB1) has been reported to be a potent proangiogenic factor induced by inflammatory stress. In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. METHODS The VEGF-A protein and mRNA levels in conditioned...

INTRODUCTION This study investigated the inhibition of the antimicrobial activity of sodium hypochlorite (NaOCl) by bovine serum albumin (BSA). The killing of Enterococcus faecalis, Candida albicans, Staphylococcus epidermidis, and Escherichia coli by NaOCl in concentrations from 2% to 0.03% was measured in the presence of BSA in concentrations between 6.7% and 0.1%. METHODS NaOCl, BSA, and m...

Passage of bovine serum albumin (BSA) into unimplanted rabbit blastocysts was studied using intravenous or intrauterine injection of the mother and in vitro cultivation of 5-day blastocysts. BSA concentration in blastocyst fluid was measured using a quantitative radial immunodiffusion method and confirmed by double diffusion in agar. Intravenous injection of up to 200 mg/kg body weight produced...

The interaction of bllirubin at concentrations less than 25 PM with bovine serum albumin (BSA) in aqueous solutions ranging from pH 3.5 to 8.6 was studied at room temperature by using the methods of optical rotatory dispersion (ORD) and absorption spectrophotometry between 230 and 600 mp. The binding of bilirubin by BSA at pH 5 caused a red shift of an absorption maximum from about 440 to 457 r...

the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...

This paper describes the conditions of preparation of poly(o-caprolactone) (PCL) microparticles with a mean size between 5 and 10 mm, obtained by a double emulsion-solvent evaporation technique, suitable for oral vaccine delivery. Bovine serum albumin (BSA) was used as water-soluble model antigen for encapsulation. Different parameters influencing the microparticle size, the BSA loading and ent...

Three dietary monosaccharides, (glucose, fructose, and ribose), have different rates of protein glycation that accelerates the production of advanced glycation end-products (AGEs). The present work was conducted to investigate the effect of ferulic acid (FA) on the three monosaccharide-mediated protein glycations and oxidation of BSA. Comparing the percentage reduction, FA (1-5 mM) reduced the ...

Immunological tolerance produced in rabbits by neonatal injections of BSA can be terminated by a series of injections of certain heterologous serum albumins which cross-react with BSA. Injections of albumins distantly related to BSA were more effective in terminating the tolerant state than injections of albumins closely related to BSA. It was concluded from results obtained with several hetero...

BACKGROUND AND THE PURPOSE OF THE STUDY The binding ability of a drug to serum albumin has influence on the pharmacokinetics of a drug. In the present study, the mutual interaction of anticancer drug erlotinib hydrochloride with bovine serum albumin (BSA) using fluorescence and UV/vis spectroscopy was investigated. METHODS The BSA solution (0.1 mM) was prepared daily in Tris buffer (0.05 mol ...