Jojoba (Simmondsia chinensis (Link) Schn.) is a nontraditional crop in arid and semi-arid areas. Vegetative propagation can be achieved by layering, grafting, or rooting semi-hardwood cuttings, but the highest number of possible propagules is limited by the size of the plants and time of the year. Micropropagation is highly recommended strategy for obtaining jojoba elite clones. For culture ini...

Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

Nodal segments (1.5 3.0 cm long) of six promising jojoba genotypes were cultured in vitro on solidified MS medium supplemented with BA in combination with different concentrations of NAA, IAA or IBA for shoot formation. The plant growth regulators combination of 5.55 μM BA + 7.1 μM IAA proved the best for shoot initiation and subsequent growth. Some explants of the jojoba genotypes, PKJ-3 and P...

In the commercial production of roses, introducing a method to reduce the time of propagation with maximum success is crucial. This study was conducted to investigate the effect of different concentrations (0, 1500, 3000, 4500 mg/L) of Indole-3-butyric acid (IBA) on propagation of three cultivars of Rosa hybrid L. ('Dolce Vita', 'Samurai' and 'Utopia') by stenting (simultaneous cutting and graf...

two experiments were performed for in vitro establishment, proliferation and shoot growth in axillary bud explants of sebri pear cultivar. in the first experiment, the effect of different concentrations of iba(indole-3-butyric acid) on explants’ establishment was evaluated. in a second experiment, the influence of several combinations of pgrs (plant growth regulators)on shoot proliferation and ...

Purpose: Rauwolfia serpentina (L.) Benth, from the family, Apocynaceae, is an important medicinal plant due to the alkaloid content of its root. The purpose of this study was to obtain roots directly from leaf explant using growth regulators. Methods: The leaf explant was inoculated on MS (Murashige and Skoog) medium supplemented with single and combinations of growth regulators. Root growth wa...

The micropropagation of Stevia rebaudiana using stem node segment explants obtained from 2 years-old shrubs were achieved. Cultures were established on Murashige and Skoog (MS) as the medium supplemented with different concentrations of 6-benzylaminopurine (BA) at concentration, 0.0, 0.1 and 0.5 mg/l individually or in combination with Kinetin (Kin) at concentration, 0.0, 0.1 and 0.5 mg/l. The ...

Clerodendrum pholomidis is an important medicinal plant of Lamiaceae. Leaves, internode and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of 2, 4-dichlorophenoxy acetic acid (2, 4-D), kinetin (kn) and indole-3 butyric acid (IBA). Leaf explants formed clusters of globular somatic embryos directly from epidermal cells within 12-15d when cultured ...

An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Co7. Shoot bud induction occurred on culturing epicotyl segments in MS (Murashige and Skoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. Adventitious buds were induced on MS medium supplemented with varying concentration of thidiazuron (TDZ: 0.5-10 μ...