نتایج جستجو برای: 3d culture

تعداد نتایج: 461478  

Journal: :Biomedical optics express 2016
F Momey A Berdeu T Bordy J-M Dinten F Kermarrec Marcel N Picollet-D'hahan X Gidrol C Allier

New microscopes are needed to help realize the full potential of 3D organoid culture studies. In order to image large volumes of 3D organoid cultures while preserving the ability to catch every single cell, we propose a new imaging platform based on lensfree microscopy. We have built a lensfree diffractive tomography setup performing multi-angle acquisitions of 3D organoid culture embedded in M...

Journal: :Assay and drug development technologies 2014
Rasheena Edmondson Jessica Jenkins Broglie Audrey F Adcock Liju Yang

Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to their evident advantages in providing more physiologically relevant information and more predictive data for in vivo tests. In this review, we discuss the characteristics of 3D cell culture systems in comparison to the two-dimensional (2D) monolayer culture, focusing on ce...

2016
Xuan Zhang Ming-Gen Hu Ke Pan Chong-Hui Li Rong Liu

Three-dimensional (3D) cell culture has been reported to increase the therapeutic potentials of mesenchymal stem cells (MSCs). However, the action mechanisms of 3D MSCs vary greatly and are far from being thoroughly investigated. In this study, we aimed to investigate the therapeutic effects of 3D spheroids of human adipose-derived MSCs for hepatic fibrosis. Our results showed that 3D culture e...

Journal: :Journal of cell science 2010
Srivatsan Raghavan Colette J Shen Ravi A Desai Nathan J Sniadecki Celeste M Nelson Christopher S Chen

We present a novel microfabricated platform to culture cells within arrays of micrometer-scale three-dimensional (3D) extracellular matrix scaffolds (microgels). These microscale cultures eliminate diffusion barriers that are intrinsic to traditional 3D culture systems (macrogels) and enable uniform cytokine stimulation of the entire culture population, as well as allow immunolabeling, imaging ...

Journal: :iranian journal of basic medical sciences 0
sajad sahab negah histology and embryology group, basic science department, faculty of veterinary medicine, shiraz university, shiraz, iran shefa neuroscience research center, khatam alanbia hospital, tehran, iran hadi aligholi department of neuroscience, school of advanced medical sciences and technologies, shiraz university of medical sciences, shiraz, iran zabihollah khaksar histology and embryology group, basic science department, faculty of veterinary medicine, shiraz university, shiraz, iran hadi kazemi pediatric department, medical faculty, shahed university, tehran, iran sayed mostafa modarres mousavi shefa neuroscience research center, khatam alanbia hospital, tehran, iran

objective(s): in order to grow cells in a three-dimensional (3d) microenvironment, self-assembling peptides, such as puramatrix, have emerged with potential to mimic the extracellular matrix. the aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma st...

Journal: :modares journal of medical sciences: pathobiology 2010
nader vazifeh shiran masoud soleimani yousef mortazavi saeed kaviani hassan aboulghasemi

objective: allogeneic transplantation with umbilical cord blood (ucb) in adult recipients is limited mainly by a low cd34+ cell dose. to overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (mpc)- unrestricted somatic stem cells (ussc)- was incorporated in an attempt to expand cd34+ cells from ucb. to provide a similar environment in vitro, we coated ...

Journal: :International journal of oncology 2014
Phanthakarn Tit-Oon Daranee Chokchaichamnankit Amnart Khongmanee Phannee Sawangareetrakul Jisnuson Svasti Chantragan Srisomsap

Cholangiocarcinoma (CCA) is a lethal malignancy which occurs with relatively high incidence in Thailand. This cancer is often difficult to diagnose and associated with high mortality. The secretome, containing the secreted proteins from cells, are potentially useful as biomarkers of cancers. Since three-dimensional (3D) cell culture may mimic growth characteristics and microenvironment of solid...

Journal: :Breast Cancer Research 2011

2014
Yang Shi Jingyu Wang Huaimin Wang Yanhui Hu Xuemei Chen Zhimou Yang

A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine Serum (FBS) within minutes. The hydrogel was characterized by rheology, transmission electron microscopy, and fluorescence emission spectra. Their...

2016
Kristin Stock Marta F. Estrada Suzana Vidic Kjersti Gjerde Albin Rudisch Vítor E. Santo Michaël Barbier Sami Blom Sharath C. Arundkar Irwin Selvam Annika Osswald Yan Stein Sylvia Gruenewald Catarina Brito Wytske van Weerden Varda Rotter Erwin Boghaert Moshe Oren Wolfgang Sommergruber Yolanda Chong Ronald de Hoogt Ralph Graeser

Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and validation. Here, 2D culture and 3D mono- and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines:...

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