nloaded s-of-function mutations of retinoblastoma family (Rb) proteins drive tumorigenesis by overcoming baro cellular proliferation. Consequently, factors modulating Rb function are of great clinical import. Here, ow that miR-335 is differentially expressed in human cancer cells and that it tightly regulates the exon of Rb1 (pRb/p105) by specifically targeting a conserved sequence motif in its...

Downlo s-of-function mutations of retinoblastoma family (Rb) proteins drive tumorigenesis by overcoming baro cellular proliferation. Consequently, factors modulating Rb function are of great clinical import. Here, ow that miR-335 is differentially expressed in human cancer cells and that it tightly regulates the exon of Rb1 (pRb/p105) by specifically targeting a conserved sequence motif in its ...

OBJECTIVE MiRNAs act as gene silencers that are involved in the regulation of essential cell functions. miR-335 is involved in regulating cell differentiation processes in progenitor cells. Mesenchymal stem cells (MSCs) are progenitor cells of chondrocytes and osteoblasts responsible for homeostatic maintenance of cartilage and bone. The aim of this study was to determine a possible relationshi...

We studied the role of carboxyl tail cysteine residues and their palmitoylation in constitutive signaling by the thyrotropin-releasing hormone (TRH) receptor type 1 (TRH-R1) in transfected mammalian cells and in Xenopus laevis oocytes. To study palmitoylation, we inserted a factor Xa cleavage site within the third extracellular loop of TRH-R1, added a carboxyl-terminal C9 immunotag and expresse...

Paired box 6 (PAX6), a highly conserved transcription factor, is important in glioma. However, the molecular mechanisms involved remain unclear. The present study demonstrated that the expression of PAX6 was significantly reduced with the malignancy of glioma and also identified PAX6 as a novel target of microRNA (miR)‑335, which was significantly upregulated in glioma. The inhibition of miR‑33...

MiRNAs can have pleiotropic effects by targeting multiple genes belonging to diverse signalling networks. Alternatively, miRNAs can enhance the potency of their cellular effects by targeting multiple genes within the same genetic pathway. Previously, we and others have demonstrated that miR-335 is a potent suppressor of tumour cell migration, invasion and metastasis, in part by targeting severa...

MicroRNAs contribute to the maintenance of optimal cellular functions by fine-tuning protein expression levels. In the pancreatic β-cells, imbalances in the exocytotic machinery components lead to impaired insulin secretion and type 2 diabetes (T2D). We hypothesize that dysregulated miRNA expression exacerbates β-cell dysfunction, and have earlier shown that islets from the diabetic GK-rat mode...

Two parameters are commonly used for the evaluation of antibiotic efficacy in animal models: bacterial counts in tissue fluids and survival rate. The second parameter is easy to assess and seems more relevant from the clinical perspective; a curative dose is used as a reliable end-point for showing subtle differences in antibiotic behaviour. Reduction in bacterial counts provides information on...

These supplemental notes for the course CAAM 335 Matrix Analysis are, as the name suggests, meant to be used in combination with the lectures and with the CAAM 335 course notes. They contain write-ups of some of the material discussed in class as well as several additional examples. You get the most out of these notes if you look through the relevant sections before each class (to recognize wha...

BACKGROUND AND OBJECTIVES The diagnostic yield of push enteroscopy (PE) varies widely from 13 to 78% of cases, according to the various series. The aim of this retrospective cohort study was to determine the endoscopic and histological yield of PE in our health area. PATIENTS AND METHODS A total of 355 consecutive patients (190 males/165 females; mean age 45 years, range 15-89) underwent PE o...