Versican-like proteoglycans are the main component of the intimal extracellular matrix interacting with low density lipoprotein (LDL). The aim of this study has been to investigate the receptors involved in versican-modified LDL uptake by human vascular smooth muscle cells (VSMCs). We have found that versican-LDL interaction leads to the following: (1) monomeric LDL particles that are similar i...

Twenty-four adult male African green grivet monkeys were fed diets containing 42% of calories as lard or menhaden oil and 0.76 mg of cholesterol/kcal for a period of 8 months. Plasma samples from fasting animals were then taken and low density lipoproteins (LDL) were isolated by ultracentrifugation and agarose column chromatography. The LDL were analyzed chemically, and physical properties of t...

Low density lipoprotein (LDL), the major cholesteroltransport protein in human plasma, binds to specific receptors on mammalian cells and is taken up by receptor-mediated endocytosis and digested in lysosomes, thereby delivering cholesterol to the cells. In the current paper, we establish conditions in which the growth of Chinese hamster ovary (CHO) cells and the Raji line of human malignant ly...

OBJECTIVE To determine whether n-3 fatty acids (n-3) influence arterial cholesterol delivery and lipoprotein lipase (LpL) levels in insulin-resistant mice. METHODS AND RESULTS Insulin resistance contributes to risk of cardiovascular disease. It was previously reported that saturated fat (SAT) diets increased, but n-3 diets decreased, arterial low-density lipoprotein (LDL) cholesterol depositi...

We determined the effects of dietary n-6 and n-3 polyunsaturated fatty acids (PUFA) on parameters of plasma lipoprotein and hepatic lipid metabolism in LDL receptor (LDLr) knockout mice. Dietary n-3 PUFA decreased the rate of appearance and increased the hepatic clearance of IDL/LDL resulting in a marked decrease in the plasma concentration of these particles. Dietary n-3 PUFA increased the hep...

We studied the influence of glucagon on hepatic LDL receptors and plasma lipoproteins in rats. A dose-dependent (maximum, threefold) increase in LDL-receptor binding was evident already at a dose of 2 x 4 micrograms, and detectable 3 h after injection; concomitantly, cholesterol and apolipoprotein (apo) B and apoE within LDL and large HDL decreased in plasma. LDL receptor mRNA levels were howev...

The low density lipoprotein (LDL) receptor has been purified to homogeneity from rabbit liver by a combination of DEAE-Sephacel chromatography, LDL-Sepharose 4B chromatography and preparative SDS/polyacrylamide-gel electrophoresis. The receptor protein had a pI of 4.45 and an Mr of 120 x 10(3)-125 x 10(3) in SDS gels under non-reducing conditions. Incubation of the LDL receptor with neuraminida...

Overweight and obesity is a major health problem in throughout the world. Hyperlipidemia are risk factor occurrence of cardiovascular disease. Exercise recommended as therapeutic lifestyle changes because it leads to variety benefits. Fun Aerobic gymnastics aerobics one which quite popular community gym. Describe fun aerobic workout's effectiveness against related prevention disease with seeing...

This article is available online at http://www.jlr.org An increased low-density lipoprotein cholesterol (LDL-C) level is an established risk factor for cardiovascular disease. Statins (3-hydroxy-3-methylglutaryl CoA reductase inhibitors) effectively reduce LDL-C levels and the risk of cardiovascular events ( 1 ). Statins also diminish infl ammation as indicated by a reduction of C-reactive prot...