Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated ...

Sucrose density gradient analyses of pH 5.5 and pH 7.4 extracts from rat liver nucleoli revealed the presence of two broad peaks of approximately 60S and 80S, and 60S and 80-100S, respectively. Ribonucleoprotein (RNP) particles containing precursor ribosomal RNA in these peaks have been characterized by electron microscopy and RNA analyses. Spherical particles only were found in the 60S peak of...

We have examined the time course and patterns of covalent aflatoxin B1:DNA adducts produced within the ribosomal RNA gene sequences isolated from the liver nuclear DNA of aflatoxin B1-treated animals. Liver nuclear DNA was initially enriched in ribosomal DNA by cesium salt density centrifugation, and incubated under alkaline conditions to stabilize bound aflatoxin B1-DNA moieties. Alkali-treate...

We have examined the time course and patterns of covalent aflatoxin B^DNA adducts produced within the ribosomal RNA gene sequences isolated from the liver nuclear DNA of aflatoxin Bi-treated animals. Liver nuclear DNA was initially enriched in ribosomal DNA by cesium salt density centrifugaron, and incu bated under alkaline conditions to stabilize bound aflatoxin ST ONA moieties. Alkali-treated...

BACKGROUND Invasive aspergillosis (IA) is an important cause of morbidity and mortality in immunocompromised individuals. This study was conducted to identify a desirable target DNA sequence for the diagnosis of aspergillosis using real-time quantitative polymerase chain reaction (qPCR). METHODS Genomic DNA was extracted from Aspergillus, Candida, and bacteria species, and qPCR was applied to...

Serpulidae Rafinesque, 1815 is a speciose group of polychaetes that all inhabit calcareous tubes. The family was traditionally subdivided into Serpulinae, Filograninae, and Spirorbinae. Recent phylogenetic analyses have suggested both Filograninae Serpulinae are paraphyletic, though with limited sampling. Here we report the first analysis based on comprehensive sampling genera (though excluding...

A class of RNA in HeLa cytoplasm with a sedimentation value of 22S is described . This RNA is a true cytoplasmic component and is not an artifact of cell rupture or of the method of RNA preparation. The 22S RNA sediments in a sucrose gradient with those ribosomal structures containing 28S ribosomal RNA (60S and 74S particles and polyribosomes) . It has a base composition and methyl content simi...

The bacterial homologue of C4orf14, YqeH, has been linked to assembly of the small ribosomal subunit. Here, recombinant C4orf14 isolated from human cells, co-purified with the small, 28S subunit of the mitochondrial ribosome and the endogenous protein co-fractionated with the 28S subunit in sucrose gradients. Gene silencing of C4orf14 specifically affected components of the small subunit, leadi...