Three Japanese individuals with homozygous ‘-thalassemia from different families were the subjects of molecular genetic analysis. They were homozygous for seven polymorphic sites in the fl-globin gene cluster. Nucleotide sequence analysis of the 6-globin gene cloned from each patient revealed a single nucleotide substitution (T-C) 77 base pairs 5’ to the cap site, just upstream of the CCAAC box...

By D. Hoelzer, E. Thiel, H. L#{246}ffler, H. Bodenstein, L. Plaumann, T. B#{252}chner, D. Urbanitz, P. Koch, H. Heimpel, R. Engelhardt, U. Muller, F.-C. Wendt, H. Sodomann, H. R#{252}hl, F. Herrmann, W. Kaboth, H. Dietzfelbinger, H. Pralle, Ch. Lunscken, K.-P. Hellriegel, S. Spors, R.M. Nowrousian, J. Fischer, H. F#{252}lle, P.S. Mitrou, M. Pfreundschuh, Ch. G#{246}rg,B. Emmerich, W. Queisser, ...

The general overexpression of p53 by different types of tumor cells suggests that p53 immunity might be generally useful for tumor immunotherapy. We describe here the induction of immunity to p53 and resistance to tumor metastasis using an idiotypic network. Mice were immunized with domain-specific anti-p53 monoclonal antibodies (Ab1): PAb-248 directed to the N-terminus; PAb-246 directed to the...

ST-246 (Tecovirimat) is a small synthetic antiviral compound being developed to treat pathogenic orthopoxvirus infections of humans. The compound was discovered as part of a high throughput screen designed to identify inhibitors of vaccinia virus-induced cytopathic effects. The antiviral activity is specific for orthopoxviruses and the compound does not inhibit the replication of other RNA- and...

Dystrophin has been shown to be associated in cells with actin bundles. Dys-246, an N-terminal recombinant protein encoding the first 246 residues of dystrophin, includes two calponin-homology (CH) domains, and is similar to a large class of F-actin cross-linking proteins including alpha-actinin, fimbrin, and spectrin. It has been shown that expression or microinjection of amino-terminal fragme...

The general overexpression of p53 by different types of tumor cells suggests that p53 immunity might be generally useful for tumor ininiunotherapy. We describe here the induction of immunity to p53 and resistance to tumor metastasis using an idiotypic network. Mice were immunized with domain-specific anti-p53 monoclonal antibodies (Abl): PAb-248 directed to the N-terminus; PAb-246 directed to t...

This paper presents a study of the role of positive charge in the P(i) binding site of Escherichia coli ATP synthase, the enzyme responsible for ATP-driven proton extrusion and ATP synthesis by oxidative phosphorylation. Arginine residues are known to occur with high propensity in P(i) binding sites of proteins generally and in the P(i) binding site of the betaE catalytic site of ATP synthase s...

the protozoan intestinal parasite cryptosporidium commonly infects cattle throughout the world and iran. the present study was undertaken to determine the abundance and associated risk factors of cryptosporidium infection in cattle herds of northwestern iran. a total number of 246 fecal samples from 138 (56.1%) diarrheic (d) and 108 (43.9%) non-diarrheic (nd) cattle were randomly collected and ...