Glutamic acid decarboxylase (GAD;E.C. 4.1.1.15) catalyzes the production of GABA, the major inhibitory neurotransmitter in the mammalian brain. We recently isolated a lambda gt-11 recombinant, lambda-GAD, that contains the cDNA for GAD from feline brain (Kaufman et al., 1986). Interestingly, the beta-galactosidase-GAD fusion protein encoded by lambda GAD is enzymatically active, catalyzing the ...

We have shown previously that chick muscle cells transformed with Rous sarcoma virus are unable to form clusters of acetylcholine receptors (AChRs) (Anthony, D. T., S. M. Schuetze, and L. L. Rubin. 1984. Proc. Natl. Acad. Sci. USA. 81:2265-2269) and are missing a 37-KD tropomyosin-like protein (TM-2) (Anthony, D. T., R. J. Jacobs-Cohen, G. Marazzi, and L. L. Rubin. 1988. J. Cell Biol. 106:1713-...

This study examined whether two machine-marked tests (MMTs; a clinical problem-solving test and situational judgement test), previously validated for selection into U.K. general practice (GP) training, could provide a valid methodology for shortlisting into core medical training (CMT). A longitudinal design was used to examine the MMTs' psychometric properties in CMT samples, and correlations b...

The European Stroke Organisation held its first European Stroke Science Workshop in Garmisch-Partenkirchen, Germany (December 15–17, 2011). Stroke experts based in Europe were invited to present and discuss their current research. The scope of the workshop was to review the most recent findings of selected topics in stroke, to exchange ideas, to stimulate new research, and to enhance collaborat...

Methods Cohort observational study including pediatric patients receiving anti-TNFa agents in a tertiary-care pediatric hospital. LTI is ruled out by the implementation of antiTNFa drugs by tuberculin skin test (TST) and, from March 2012, QuantiFERON Gold-In Tube test (QTF). Along treatment, patients are evaluated periodically for TB using history and physical examination, but TST/ QTF are not ...

Statistics courses are frequently perceived by tertiary students as extremely difficult and anxiety-inducing, negatively impacting student outcomes experiences. To address this, the present study considered worked example videos (where an instructor demonstrates solution to a problem while narrating process) blended learning approach for enhancing introductory business statistics course taken 2...

aLaboratório de Biologia Comparada de Hymenoptera, Programa de Pós-graduação em Entomologia, Universidade Federal do Paraná – UFPR, CP 19020, CEP 81531-980, Curitiba, PR, Brazil bPrograma de Pós-graduação em Biologia Comparada, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto – FFCLRP, Universidade de São Paulo – USP, Av. Bandeirantes, 3900, Monte Alegre, CEP 14040-901, Ribeirão Preto...

Numerical predictions of blood flow patterns and hemodynamic stresses in Abdominal Aortic Aneurysms (AAAs) are performed in a two-aneurysm, axisymmetric, rigid wall model using the spectral element method. Homogeneous, Newtonian blood flow is simulated under steady conditions for the range of Reynolds numbers 10 < or =Re < or =2265. Flow hemodynamics are quantified by calculating the distributi...

The European Stroke Organisation held its first European Stroke Science Workshop in Garmisch-Partenkirchen, Germany (December 15–17, 2011). Stroke experts based in Europe were invited to present and discuss their current research. The scope of the workshop was to review the most recent findings of selected topics in stroke, to exchange ideas, to stimulate new research, and to enhance collaborat...