Plasmid pRO1957 contains a 26.5-kb BamHI restriction endonuclease-cleaved DNA fragment cloned from the chromosome of Pseudomonas pickettii PKO1 that allows P. aeruginosa PAO1c to grow on toluene, benzene, phenol, or m-cresol as the sole carbon source. The genes encoding enzymes for meta cleavage of catechol or 3-methylcatechol, derived from catabolism of these substrates, were subcloned from pR...

Saturation mutagenesis was used to generate eleven substitutions of toluene-o-xylene monooxygenase (ToMO) at alpha subunit (TouA) positions F176 and F196 among which nine were novel: F176H, F176N, F176S, F176T, F196A, F196L, F196T, F196Y, F196H, F196I, and F196V. By testing the substrates phenol, toluene, and naphthalene, these positions were found to influence ToMO oxidation activity and regio...

The availability of iron (Fe) to marine microbial communities is enhanced through complexation by ligands. In Fe limited environments, measuring the distribution and identifying likely sources ligands therefore central understanding drivers productivity. Antarctic coastal environments support highly productive ecosystems are influenced numerous ligands, magnitude which varies both spatially sea...

The Henry's Law coefficient is a key physical parameter in the partitioning, and hence environmental fate, of a chemical species between air and water. Despite the acknowledged polluting potential of phenol, 2-methylphenol (o-cresol) and 2nitrophenol, there is extremely poor agreement in the literature of their Henry's law coefficients and, in particular, no apparent systematic measurement of t...