Magnesium ions (Mg2+) are divalent cations essential for various cellular functions. Mg2+ homeostasis is maintained through Mg2+ channels such as MgtE, a prokaryotic Mg2+ channel whose gating is regulated by intracellular Mg2+ levels. Our previous crystal structure of MgtE in the Mg2+-bound, closed state revealed the existence of seven crystallographically-independent Mg2+-binding sites, Mg1-Mg...

The effect of imipramine on Mg2+ efflux in NaCl medium (Na+/Mg2+ antiport), on Mg2+ efflux in choline.Cl medium (choline/Mg2+ antiport) and on Mg2+ efflux in sucrose medium (Cl- -coupled Mg2+ efflux) was investigated in rat erythrocytes. In non-Mg2+-loaded rat erythrocytes, imipramine stimulated Na+/Mg2+ antiport but inhibited choline/Mg2+ antiport and Cl- -coupled Mg2+ efflux. The same effect ...

BACKGROUND We previously reported that administration of elastase inhibitors reverses fatal pulmonary arterial hypertension (PAH) in rats by inducing smooth muscle cell (SMC) apoptosis. We showed in pulmonary artery (PA) organ culture that the mechanism by which elastase inhibitors induce SMC apoptosis involves repression of matrix metalloproteinase (MMP) activity and subsequent signaling throu...

The taxonomy of the fish genus Platycephalus Bloch, 1785 from Australia is revised following examination of all extant primary types of Australian species, including those previously synonymized. The following 16 Australian species, including a single new species, are recognized in this study: Platycephalus angustus Steindachner, 1866, Platycephalus aurimaculatus Knapp, 1987, Platycephalus bass...

We employ Schwarzschild’s method of orbit modeling to constrain the mass profiles of the central lens galaxies in 0957+561 and PG 1115+080. We combine the measured central projected stellar velocity dispersions of these galaxies with the self-similar radial profiles of the rms velocity and of the Gauss-Hermite moment h4 observed in nearby galaxies for 0 <∼ R <∼ 2Reff . For 0957+561, we find a 1...

The effects of temperature (0, 5, 10, 15 and 20 &deg;C) and of anoxia (at 5 &deg;C) on extracellular Mg2+ concentration ([Mg2+]e), intracellular pH (pHi) and ATP and lactate levels were investigated in intermoult adults of the common shrimp Crangon crangon. All animals caught in summer (summer animals) showed a slight but significant increase in [Mg2+]e at low temperatures. In contrast, at ever...

Extracellular magnesium ions [Mg2+]o are known to regulate functions of endothelial cells, but whether [Mg2+]o can alter intracellular free ionized magnesium [Mg2+]i in these cells remains unknown. The present studies, using digital imaging microscopy and the Mg2+ fluorescent probe, mag-fura-2, determined effects of elevation of [Mg2+]o on [Mg2+]i in cultured human aortic endothelial cells. Wit...