Molecular phylogeny increasingly supports the understanding of organismal relationships and provides the basis for the classification of microorganisms according to their natural affiliations. Comparative sequence analysis of ribosomal RNAs or the corresponding genes currently is the most widely used approach for the reconstruction of microbial phylogeny. The highly and less conserved primary a...

A new species of heterocytous cyanobacterium Dulcicalothrix alborzica sp. nov. (Calotrichaceae) was isolated from Iran and described following a polyphasic approach. Morphological examination indicated that the strain, initially called "Alborzica", belonged to genus Calothrix. Nonetheless, 16S rRNA gene analysis showed "Alborzica" were within cluster. In order confirm taxonomic position this ta...

The main bacteria in peaty, acid grassland soils in the Netherlands were investigated by ribosome isolation, temperature gradient gel electrophoresis, hybridization, cloning, and sequencing. Instead of using only 16S rDNA to determine the sequences present, we focused on rRNA to classify and quantify the most active bacteria. After direct ribosome isolation from soil, a partial amplicon of bact...

In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S...

Based on a 16S rRNA gene fragment, molecular phylogeny for the genus Odorrana Fei, Ye & Huang, 1990 was reconstructed, validity of poorly-known ranid species O. macrotympana (Yang, 2008) confirmed and its phylogenetic position evaluated. In addition, we report first country record from Myanmar, based our new records Htamanthi Wildlife Sanctuary, Sagaing Division present supplementary de...

The abundance and composition of bacteria of the phylum Acidobacteria were surveyed in subsurface sediments from uranium-contaminated sites using amplification of 16S rRNA genes followed by clone/sequence analysis. Analysis of sequences from this study and public databases produced a revised and greatly expanded phylogeny of the Acidobacteria phylum consisting of 26 subgroups.

The abundance and composition of Acidobacteria phylum bacteria were surveyed in subsurface sediments from uranium-contaminated sites using amplification of 16S rRNA genes followed by clone/sequence analysis. Analysis of sequences from this study and 5 public databases produced a revised and greatly expanded phylogeny of the Acidobacteria phylum consisting of 26 subgroups.

Partial sequences of the RNase P RNA gene (rnpB) were obtained from a number of hemoplasmas and other Mycoplasma species. Phylogenetic analysis of these sequences showed that all hemoplasmas were present within a single clade and were most closely related to Mycoplasma fastidiosum, similar to the results found with 16S rRNA gene phylogeny.

The proliferation of benthic cyanobacteria has been observed in Lake Baikal since 2011 and is a vivid manifestation the ecological crisis occurring littoral zone. cyanobacterium Symplocastrum sp. formed massive fouling on all types substrates, including endemic sponges. strain BBK-W-15 (=IPPAS B-2062T), which was isolated from sponge 2015, used for further taxonomic determination. A polyphasic ...