نتایج جستجو برای: 10 selected rapd primers produced 160 bands

تعداد نتایج: 1694252  

Journal: :علوم باغبانی ایران 0
بهروز سرابی دانشجوی سابق کارشناسی ارشد پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمدرضا حسندخت دانشیار پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمد اسماعیل حسنی محقق دانشکده کشاورزی، غذا و منابع طبیعی، پارک تکنولوژی دانشگاه سیدنی، استرالیا تیمور رمک معصومی مربی پردیس کشاورزی و منابع طبیعی دانشگاه تهران

rapd markers were employed to assess the genetic diversity of 34 iranian wild edible asparagus (a. officinalis) genotypes as well as cv. mary washington and a. persicus. in total, 80 rapd primers were employed for pcr reactions, among which, 18 revealed reasonable polymorphism. the 18 primers produced 175 bands, of which, 160 were polymorphic (91.4% polymorphism). cluster analysis of the genoty...

Journal: :Bionatura (Ibarra - Impresa) 2022

RAPD-PCR genetic markers were used to assess variation in wheat plants and connections among six genotypes. Four random primers produced 140 DNA fragments, averaging 6.7 identifiable bands per primer. Among the genotypes, 85 pieces (44.64 percent) polymorphic. Several RAPD marker had distinct signify recurrence patterns that thing differently amongst germplasm of groupings. Within-community acc...

2011
N Najafi Ramin Hosseini AR Ahmadi

BACKGROUND AND OBJECTIVES Phaffia rhodozyma is a red yeast which produces astaxanthin as the major carotenoid pigment. Astaxanthin is thought to reduce the incidence of cancer and degenerative diseases in man. It also enhances the immune response and acts as a free-radical quencher, a precursor of vitamin A, or a pigment involved in the visual attraction of animals as mating partners. The impac...

2014
Kusum Yadav Sanjay Kumar Yadav Anurag Yadav Veda Prakash Pandey Upendra Nath Dwivedi

Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic b...

Journal: :Genetics and molecular research : GMR 2011
D T Phong V T T Hien T T V Thanh D V Tang

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 R...

2009
Shalini Saurabh Tiwari Rajinder K Gupta Dolly Wattal Dhar

Random amplification of polymorphic DNA (RAPD) analysis using the polymerase chain reaction proved a useful technique in the biodiversity analysis of microorganisms but may lack reproducibility in the poorly standardized methodology. In the present investigation, the RAPD technique was optimized for characterizing cyanobacterial isolates in order to ensure its reproducibility and discriminatory...

Journal: :Genetics and molecular research : GMR 2015
M Korkmaz N Y Dogan

Gypsophila L. is the 3rd-largest genus of Caryophyllaceae in Turkey, a country that includes 60 taxa belonging to 56 species of the 126 recognized in the genus. A total of 35 taxa are endemic to Turkey, with an approximately 60% endemism ratio. In this study, the genetic diversity of 14 Gypsophila species from Turkey was analyzed using random amplification of polymorphic DNA (RAPD) and inter-si...

Journal: :Genetics and molecular research : GMR 2013
G P Li C Q Zhang F L Cao

Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagram that consisted of polymorphic ba...

2001
T. PRADEEPKUMAR J. L. KARIHALOO SUNIL ARCHAK

CURRENT SCIENCE, VOL. 81, NO. 3, 10 AUGUST 2001 248 by repeating the experiment at least once. A total of 34 decamer primers (series OPA, OPB etc.) from Operon Tech, CA, USA were screened using three representative genomic DNA samples of black pepper. Of these, 24 primers that yielded consistent and clear band patterns were selected for the final analysis of the 22 accessions used in the presen...

2013
P. Bertin Mary Devraja Achar

For the first time, genetic variability and differentiation among Ten Synconium white butterfly populations originating from tissue cultured plants maintained in pro-trays under the poly house & green house conditions in Indo American hybrid Seed Company, Bangalore were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity. Th...

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