نتایج جستجو برای: 10 populations including 59 genotypes via 13 rapd primers

تعداد نتایج: 2842727  

The present study was conducted to investigate genetic diversity between and within of six populations with different individual numbers of cultivated and non-cultivated provenances of  Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. using RAPD, ISSR and various combinations of RAPD and ISSR primers in one multiplex PCR (R-ISSR). 10 primers that produced clear and ...

2012
Noha A. Rezk Hoda Mansour Nahed H. Ghoneim Mahmoud M. Rifaat

PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) has been used widely for genome identification. In this study, 13 Salmonella Typhi strains were isolated from typhoid patients from Aswan, Cairo, Fayoum, and Monofya Governorates of Egypt. The isolates, along with three reference strains, i.e., O901, H901, and Ty2 were subjected to whole genome typing by RAPD PCR. Three RAPD...

2006
P. JAYASANKAR

A holistic approach, combining one phenotypic and two genotypic methods, was adopted to analyze possible population differences in Indian mackerel (Rastrelliger kanagurta) from selected centers in the East and West coasts of India. Principal component analysis of truss landmark variables revealed that the area encompassing depth between the origin of anal and origin of second dorsal and caudal ...

2013
S. M. FAISAL M. S. HAQUE K. M. NASIRUDDIN M. M. ISLAM M. ASHRAFUZZAMAN M. A. ALI

Genetic variability among the genotypes of any species could be utilized for its improvement. PCR-based Random Amplified Polymorphic DNA (RAPD) technique was used to determine the genetic diversity and relationship among 10 cucumber varieties and genotypes. Five decamer primers were used to amplify genomic DNA and the primers yielded a total of 54 bands of which 36 bands were polymorphic and 18...

Journal: :Genetics and molecular research : GMR 2011
S Ercisli J Gadze G Agar N Yildirim Y Hizarci

The pomegranate has been used traditionally in Coruh Valley in Turkey for a long time; fruits are harvested from wild, semi-domesticated and cultivated trees. In the valley, it occurs in general along with olive trees. We sampled 23 wild-grown pomegranate genotypes sampled from different parts of Coruh Valley and compared them using RAPD primers to determine genetic variability. Eighty-six RAPD...

ژورنال: علوم آب و خاک 2009
راوش, رودابه, زرواگیس, جورج, شیران, بهروز, علوی, عزیزاله,

In this study, 45 Pleurotus isolates with a wide range of geographical origins (in five provinces of Iran: Isfahan, Chaharmahal Bakhtiary, Kohkiloye boyrahmad, Lorestan and Fars) were collected. A random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess the genetic diversity in ten populations of the Pleurotus eryngii complex. The use of ten primers allowed the p...

Journal: :Genetics and molecular research : GMR 2015
M F Santos K J Damasceno-Silva M A Carvalhaes P S C Lima

The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using random-amplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD primers. A total of 146 bands were generated, ...

2010
S. Das R. C. Misra

Genetic diversity among fifteen finger millet genotypes was estimated using RAPD markers. Out of the initial twenty-five random primers, nine RAPD primers were found to be highly reproducible and produced a total of 60 loci of which 51 loci were polymorphic. Primer OPC18 amplified highest number of polymorphic bands. Percentage of polymorphism varied from 50% to 100% with an average of 80.8%. P...

2016
Hirdayesh Anuragi Haresh L. Dhaduk Sushil Kumar Jitendra J. Dhruve Mithil J. Parekh Amar A. Sakure

Understanding the genetic variation in germplasm is of utmost importance for crop improvement. Therefore, efforts were made to analyse the molecular marker based genetic diversity of 20 Annona genotypes from five different species of family Annonaceae. During analysis, a set of 11 RAPD primers yielded a total of 152 bands with 80.01 % polymorphism and PIC for RAPD ranged from 0.86 to 0.92 with ...

2012
Ghorban Elyasi Zarringhabaie Arash Javanmard Ommolbanin Pirahary

The objective of present study was identification of genetic similarity between wild Iran and captive Azerbaijan Pheasant using PCR-RAPD markers. For this purpose, in overall, 28 birds were taken for DNA extraction and subsequently 15 arbitrary primers were applied for PCR-RAPD technique. After electrophoresis, five primers exhibited sufficient variability which yielded overall 65 distinct band...

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