5-Hydroxytryptamine type 2A receptors (5-HT2A) are G protein-coupled receptors that increase intracellular Ca2+ concentrations via activation of phospholipase C-beta and elevation of myo-inositol-1,4,5-triphosphate levels. In the central nervous system, these receptors are involved in regulating sleep and alertness. We now report that ethanol inhibited (IC50 = 41 mM) 5-HT2A receptor-induced Ca2...

Amantadine has been used for decades as an inhibitor of the influenza A virus M2 protein (AM2) in the prophylaxis and treatment of influenza A infections, but its clinical use has been limited by its central nervous system (CNS) side effects as well as emerging drug-resistant strains of the virus. With the goal of searching for new classes of M2 inhibitors, a structure-activity relation study b...

Neste estudo, um método simples e barato de microextração líquido-líquido dispersiva com solidificação da gota orgânica flutuante (DLLME-SFOD) foi desenvolvido para separação e especiação de Se inorgânico previamente à sua determinação espectrofotométrica. O método está baseado na formação do complexo amarelo piazoselenol entre Se(IV) e 3,3’-diaminobenzidina (DAB) seguido por sua extração em 1-...

A two-component Langmuir monolayer consisting of (perfluorobutyl)undecanol (F4H11OH) and dipalmitoylphosphatidylcholine (DPPC), a major component of pulmonary surfactants in mammals, has been investigated at the air-water interface. The binary monolayer has been systematically examined from both thermodynamic and morphological perspectives. The excess Gibbs free energy of mixing has been calcul...

Primary aliphatic alcohols from hexanol to pentadecanol were tested for their effects on the succinate-supported respiration of intact mitochondria isolated from rat liver. Alkanols were found to inhibit State 3 and uncoupled respiration. The ADP/oxygen ratios, a measure of the efficiency of oxidative phosphorylation, also were lowered, but to a lesser degree when compared on the basis of perce...

Bacterially produced volatile organic compounds (VOCs) can modify growth patterns of eukaryotic hosts and competing/cohabiting microbes. These compounds have been implicated in skin disorders and attraction of biting pests. Current methods to detect and characterize VOCs from microbial cultures can be laborious and low-throughput, making it difficult to understand the behavior of microbial popu...

This paper describes a method to direct the formation of microstructures of poly(isobenzofuran) (PIBF) by chemical vapor deposition (CVD) on chemically patterned, reactive, self-assembled monolayers (SAMs) prepared on gold substrates. We examined the growth dependence of PIBF by deposition onto several different SAMs each presenting different surface functional groups, including a carboxylic ac...

Widely used in microelectronics and optoelectronics; Gallium Arsenide (GaAs) is a III-V crystal with several interesting properties for microsystem and biosensor applications. Among these; its piezoelectric properties and the ability to directly biofunctionalize the bare surface, offer an opportunity to combine a highly sensitive transducer with a specific bio-interface; which are the two essen...

0