نتایج جستجو برای: 1 pyrroline 5 carboxylate synthetase p5cs

تعداد نتایج: 3385599  

2001
Esteban Sánchez Pablo C. Garcia Luis R. López-Lefebre Rosa M. Rivero Juan Manuel Ruiz Luis Romero

The objective of the present work was to determine the impact of nitrogen deficiency on proline metabolism in French Bean plants (Phaseolus vulgaris L. cv. Strike). The nitrogen was applied to the nutrient solution in the form of NH4NO3 at 1.45 mM (N1), 2.90 mM (N2) and 5.80 mM (N3, optimal level). Our results indicate N deficiency is characterised by a decline in proline accumulation both in t...

Journal: :Plant physiology 1999
A P Stines D J Naylor P B Høj R van Heeswijck

Mature fruit of grapevine (Vitis vinifera) contains unusually high levels of free proline (Pro; up to 24 micromol or 2.8 mg/g fresh weight). Pro accumulation does not occur uniformly throughout berry development but only during the last 4 to 6 weeks of ripening when both berry growth and net protein accumulation have ceased. In contrast, the steady-state levels of both the mRNA encoding V. vini...

Ferdos Rastgar-Jazii Reza Amiri Seyed Ahmad Sadat-Noori Seyed Amir Atarodi Seyed Mohammad Mahdi Mortazavian

Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1980
R J Smith S J Downing J M Phang R F Lodato T T Aoki

Although glutamic acid is known to be a precursor for proline biosynthesis, the enzymatic conversion of glutamic acid to pyrroline-5-carboxylic acid, the immediate precursor of proline, has not been demonstrated in cell-free systems. By providing appropriate concentrations of ATP and NADPH and blocking further metabolism of pyrroline-5-carboxylic acid, we have developed a method for measuring t...

Journal: :journal of cell and molecular research 0
ali akbar ehsanpour somayyeh zarei jalil abbaspour

in this study proline content and activity of catalase (cat), and ascorbate peroxidase (apx) and level of lipid peroxidation in terms of malondialdehyde (mda) content were measured in transgenic tobacco (nicotiana tabacum cv. wisconsin), over expressing a δ-1-pyrroline-5-carboxylate synthase (p5cs) gene, and non transgenic plants as control. drought stress was applied using polyethylene glycol ...

Journal: :Molecular cancer research : MCR 2015
Gregory R Kardos Hannah C Wastyk Gavin P Robertson

UNLABELLED Many processes are deregulated in melanoma cells and one of those is protein production. Although much is known about protein synthesis in cancer cells, effective ways of therapeutically targeting this process remain an understudied area of research. A process that is upregulated in melanoma compared with normal melanocytes is proline biosynthesis, which has been linked to both oncog...

Journal: :The Journal of biological chemistry 1959
T YURA H J VOGEL

Previous studies (1, 2) with mutant and wild type strains of Neurospora crassa have pointed to glutamic y-semialdehyde and its spontaneously cyclized form, Al-pyrroline-5-carboxylate, as intermediates in the biosynthesis of proline. These studies, based largely on experiments in vivo, have recently been substantiated through work in vitro with extracted enzymes (3, 4). ‘The terminal step in pro...

2005
Thomas LEISINGER

y-Glutamyl phosphate reductase, the second enzyme of proline biosynthesis, catalyses the formation of L-glutamic acid 5-semialdehyde from y-glutamyl phosphate with NAD(P)H as cofactor. It was purified 150-fold from crude extracts of Pseudomonas aeruginosa PAO 1 by DEAE-cellulose chromatography and hydroxyapatite adsorption chromatography. The partially purified preparation, when assayed in the ...

Journal: :Journal of Clinical Investigation 1981

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