Site-directed mutagenesis was applied to modify phenylalanines (Phe(475)Trp, Phe(548)Tyr, and both) to generate mutants on the basis of molecular modeling of the ATP-binding domain of Na(+)/K(+)-ATPase, in order to characterize the forces that stabilize ATP in its binding pocket. Each of the mutants was examined by Raman difference spectroscopy, i.e., as a difference between the spectrum of the...

Born-Oppenheimer approximation Hylleraas variational calculations with up to 7034 expansion terms are reported for the 1sigma(g)+ ground state of neutral hydrogen at various internuclear distances. The nonrelativistic energy is calculated to be -1.174 475 714 220(1) hartree at R = 1.4 bohr, which is four orders of magnitude better than the best previous Hylleraas calculation, that of Wolniewicz...

CONTENTS PAGE Introduction 465 A definition and some general principles 468 Neuromuscular paradigms of neurotrophic competition 471 Innervation of smooth muscle 471 Innervation of skeletal muscle 472 Indirect evidence for spatial competition in skeletal muscle 473 Predictions regarding plasticity of smooth and skeletal muscle innervation 474 Competition versus co-existence of motor nerve termin...

1Consiglio Nazionale delle Ricerche, Istituto di Metodologie per l’Analisi Ambientale (CNR-IMAA), C.da S. Loja, Potenza 85050 Tito Scalo, Italy 2 Science Systems and ApplicationS Inc., c/o NASA Langley Research Center MS 475, Hampton, VA 23681, USA 3Leibniz Institute for Tropospheric Research (IfT), Permoserstraße 15, 04318 Leipzig, Germany 4Gwangju Institute of Science and Technology (GIST), 1...

Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 47...

There are over 80 psoriasis-associated loci identified, with a majority playing regulatory roles; however, their gene targets yet to be identified. Chromatin loops play an important role in regulation, and can detected by chromosome conformation capture techniques like Hi-C. In this study, we deep sequenced Hi-C libraries (∼1 billion reads/reaction) generate nine PBMC-derived subsets from 2 ind...