The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient fo...

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates not introduced feedstocks oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder c...

The transcription initiation site of the Saccharomyces cerevisiae PRS1 gene was mapped at -179 bp. Measurement of beta-galactosidase activity of the successively deleted PRS1 promoter linked to lacZ and integrated at the ura3 locus defined three DNA regions involved in the control of PRS1 expression. Gel shift analysis confirmed the data.

(shaded bars; left scale) are presented beside β-gal activity units determined on the indicated strains in the same conditions by ONPG-based assays (empty bars; right scale), performed using the traditional Miller method for bacteria (3) and crude protein extracts for yeast (1). MUG, 4-methylumbelliferyl β-d-galactopyranoside; ONPG, o-nitrophenyl-β-d-galactopyranoside; β-gal; β-galactosidase. B...

MyoD is a muscle-specific transcription factor involved in commitment of cells to myogenesis. MyoD mRNA levels differ between fast and slow muscles, suggesting that MyoD may regulate aspects of fibre type. Here we show that detectable MyoD protein becomes restricted during development to the nuclei of the fastest classes of fibres in fast muscles. myoDm1 mice, in which the myoD gene has been di...

Background: Anderson–Fabry disease (AFD) is an X-linked that results from reduced activity of the enzyme galactosidase alpha (GLA). When GLA gene sequence altered by mutations alter normal DNA sequence, variants alpha-galactosidase A are produced, which may or not function. These responsible for Fabry disease, and to date, over 800 different have been described in patients with disease. In this...

β-Galactosidase encoded by the Escherichia coli lacZ gene, is widely used as a reporter molecule in molecular biology in a wide variety of animals. β-Galactosidase retains its enzymatic activity in cells or tissues even after fixation and can degrade X-Gal, a frequently used colormetric substrate, producing a blue color. Therefore, it can be used for the activity staining of fixed tissues. Howe...

Ehrlich ascites tumor cells and ascitic fluid were assayed for glycosidase activity. cu-Galactosidase and P-galactosidase, (Yand @-mannosidase, a-N-acetylgalactosaminidase, and /3-N-acetylglucosaminidase activities were detected using p-nitrophenyl glycosides as substrates. a-Galactosidase and a-N-acetylgalactosaminidase were isolated from Ehrlich ascites tumor cells on t-aminocaproylgalactosyl...

A rapid coliphage detection assay was developed, based on the phage-induced release of beta-galactosidase from cells of Escherichia coli. The assay could detect as few as five coliphage per sample without an overnight incubation period. The range of acceptable assay parameters was identified.

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