Methane-oxidizing bacteria (MOB) have a large potential as a microbial sink for the greenhouse gas methane as well as for biotechnological purposes. However, their application in biotechnology has so far been hampered, in part due to the relative slow growth rate of the available strains. To enable the availability of novel strains, this study compares the isolation of MOB by conventional dilut...

Screening using a reporter under the control of the hypoxia-response element (HRE) identified several flavonoids and homoisoflavonoids that inhibit the activation of HRE under hypoxic conditions. Among various compounds, isorhamnetin, luteolin, quercetin, and methyl ophiopogonanone B (MOB) were effective at 3 to 9 microg/ml in inhibiting the reporter activity. The expression of vascular endothe...

Simultaneous recordings of main olfactory bulb (MOB) and anterior piriform cortex (aPCX) neuron responses to repeated and prolonged odor pulses were examined in freely breathing, urethan-anesthetized rats. Comparisons of odor responses were made between multi-unit recordings of MOB activity and single-unit extracellular and intracellular recordings of Layer II/III aPCX neurons. Odor stimuli con...

The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica (R. Antoine and C. Locht, Mol. Microbiol. 6:1785-1799, 1992). Plasmid pBBR1 consists of two functional cassettes and presents sequence similarities with the transfer origins of several plasmids and mobilizable transpos...

Ultrahigh stocking density (113,000 kg of beef per ha to as high as 1 million kg per ha) or mob grazing has been suggested to build soil, increase forage production and plant diversity, and improve grazing distribution compared to less intensive grazing systems. Experimental evidence does not completely support such conclusions. The overall focus of this research is based on the approach of bui...

Software Development and Quality Assurance From Agile Development to DevOps: Going Towards Faster Releases at High Quality – Experiences from an Industrial Context . . . . . . . . . . . . . . . . 33 Frank Elberzhager, Taslim Arif, Matthias Naab, Inge Süß, and Sener Koban Learning to Rank Extract Method Refactoring Suggestions for Long Methods. . . . . . . . . . . . . . . . . . . . . . . . . . ....

BACKGROUND The low engraftment rate of stem/progenitor cells infused via the intracoronary route to the ischemic myocardium is one of the most important factors limiting the efficacy of cell therapy. We investigated the concept of priming peripheral blood stem cells enriched by granulocyte colony-stimulating factor mobilization and apheresis ((mob)PBSCs) with angiopoietin-1 (Ang1), to enhance t...