The NS3 protein of hepatitis C virus (HCV) possesses protease activity responsible for the proteolytic cleavage of the viral polyprotein at the junctions of nonstructural proteins downstream of NS3. The NS3 protein was also found to be internally cleaved. In this study, we demonstrated that internal cleavages occurred on the NS3 protein of genotype 1b in the presence of NS4A, both in culture ce...

Background: The yellow mealworm beetle, Tenebrio molitor, is a promising alternative protein source for animal and human nutrition its farming involves relatively low environmental costs. For these reasons, industrial scale production started this century. However, to optimize breed sustainable new T. molitor lines, t...

Background Directly Observed Treatment (DOT) is a requirement in the management of Tuberculosis (TB) globally. With transition from alternate day treatment to daily India, monitoring adherence through DOT logistic challenge. The pervasiveness mobile phones India provides unique opportunity address this challenge remotely. This ...

Background: During pregnancy, the placenta is an extremely important organ as it secretes its own hormones, e.g. insulin-like growth factor 1 (IGF-1), to ensure proper intrauterine fetal and development. Ethanol, addictive widely used drug, has numerous adverse effects during including restriction (FGR). To date, molecular mechanisms by which...

Background: Bovine congestive heart failure (BCHF) has become increasingly prevalent among feedlot cattle in the Western Great Plains of North America with up to 7% mortality affected herds. BCHF is an untreatable complex condition involving pulmonary hypertension that culminates right ventricular and death. Genes associated have not been previously identified. Our a...

Nucleostemin 3 (NS3) is an evolutionarily conserved protein with profound roles in cell growth and viability. Here we analyze cell-autonomous and non-cell-autonomous growth control roles of NS3 in Drosophila and demonstrate its GTPase activity using genetic and biochemical assays. Two null alleles of ns3, and RNAi, demonstrate the necessity of NS3 for cell autonomous growth. A hypomorphic allel...

Background: Uncaria gambir is one Uncaria species that exclusively grows in Indonesia. The phytochemical constituents of this have been widely explored and its extracts are used as traditional medicine. However, the relationship between Uncaria gambir other still unknown. DNA barcoding was stu...

Background: When moved orthodontically, hypofunctional teeth will have a decreased tooth movement rate compared to normal teeth. Hypofunctional would less VEGF expression and heparan sulfate proteoglycan production during orthodontic movement. This study aimed determine the number of osteoblasts in tension side osteoclasts pressure Method: 18 mal...

Non-structural protein 3 (NS3) is a multifunctional enzyme possessing serine protease, NTPase, and RNA unwinding activities that are required for hepatitis C viral (HCV) replication. HCV non-structural protein 4A (NS4A) binds to the N-terminal NS3 protease domain to stimulate NS3 serine protease activity. In addition, the NS3 protease domain enhances the RNA binding, ATPase, and RNA unwinding a...

Mosquito-borne WNV (West Nile virus) is an emerging global threat. The NS3 proteinase, which is essential for the proteolytic processing of the viral polyprotein precursor, is a promising drug target. We have isolated and biochemically characterized the recombinant, highly active NS3 proteinase. We have determined that the NS3 proteinase functions in a manner that is distantly similar to furin ...