The applicability of a new technique, Microwave-Accelerated Surface Plasmon-Coupled Luminescence (MA-SPCL) for fast and sensitive bioassays in buffer, serum and whole blood using quantum dots as luminescence reporters is demonstrated. In this regard, a model bioassay based on the well-known interactions of biotin and streptavidin is used. Using MA-SPCL, the bioassay was kinetically completed wi...

The effects of catecholamines (dopamine, adrenaline, noradrenaline and its derivatives), 5-hydroxytryptamine and purines (adenosine, ATP and their derivatives) on the acetylcholine-induced luminescence of isolated arms and dissociated photocytes of the luminescent ophiuroid Amphipholis squamata were tested. The results showed that catecholamines and 5-hydroxytryptamine (10(-)(5) to 10(-)(3 )mol...

All luminous animals require oxygen for luminescence bu t it is not known whether they give off CO2 as a result of the luminescence. Luminous bacteria produce carbon dioxide but this is no doubt the carbon dioxide of respiration. Even if we could prove that luminous bacteria produced more carbon dioxide than certain non-luminous bacteria, this would not necessarily znean that the excess carbon ...

The aim of the study was to evaluate the effect of semen storage on ferrous ion induced luminescence of boar spermatozoa and to determine the relationship between parameters of this luminescence and lipid peroxidation as measured by malondialdehyde (MDA) contents. Boar semen samples were diluted in Biosolwens extender and stored for 12 days at 15 degrees C. Luminescence and MDA were measured di...

The effect of a series of redox indicators and systems has been tested with a suspension of luminous bacteria (B. fischeri) in M/4 phosphate buffer of P(H) = 7.6. The indicators behave as expected from their position in the redox series, the most positive being reduced rapidly even in presence of air and before luminescence of the bacteria disappears, those of intermediate position at the time ...

Rapid and reliable in vitro methods for the detection of pathogenic leptospires, such as Leptospira interrogans, are lacking. The present study investigated the use of luminescence to replace the existing enumeration techniques. Transposon TnSC189 was modified to incorporate the luxCDABE cassette from Photorhabdus luminescens and was used to construct luminescent Leptospira spp. There was a lin...

Leiognathids are shallow-water, Indo-West Pacific fishes that have a circumesophageal, bacterial light organ. Visual observations of living fishes revealed a mottled ventral luminescence pattern, which was analyzed both behaviorally and morphologically. In behavioral experiments, these fishes responded to increases in intensity of downwelling light with increases in the intensity of ventral lum...

A simple and facile strategy was developed for regulating the luminescence of Tb(3+) sensitized by DNA, in which Ag(+) and cysteine (Cys) act as activators. The Ag(+)/Cys-mediated reversible luminescence changes in the Tb(3+)-DNA sensing system enabled the design of a DNA INHIBIT logic gate and a H2O2 sensor in a time-resolved luminescence format.

We present a model of size-dependent luminescence from a-Si:H and show that a blueshift of the luminescence energy and a general increase in luminescence quantum efficiency are predicted as structure size decreases. In contrast to bulk a-Si:H structures, highly confined a-Si:H exhibits visible luminescence peak energies and high radiative quantum efficiency at room temperature, which is insensi...