PURPOSE TAK-165 is a new potent inhibitor of human epidermal growth factor receptor 2 (HER2) tyrosine kinase. Several reports suggest HER2 expression in bladder cancer, renal cell carcinoma (RCC) and androgen-independent prostate cancer. We therefore investigated the antitumor effect of TAK-165 on these urological cancer cells. MATERIALS AND METHODS Western blot analysis was performed to conf...

CKLF-like MARVEL transmembrane domain-containing 5 (CMTM5) has been reported to function as a potential tumor suppressor in several human cancers. However, the involvement of CMTM5 in human renal cell carcinoma (RCC) remains unclear. The current study aimed to detect its expression pattern in RCC tissues and cells, and to determine its anti-proliferative functions in this malignancy. The mRNA a...

Rasfonin (A304) was originally extracted from the Taleromyces sp. and Trichurus terrophilus , and was found to have the inhibitory activity on cancer cells. However, the underlying mechanism of its regulatory role in autophagy and programmed cell death remains largely unknown. In present study, the A304-induced cell death was first detected using human renal cancer cell line ACHN. A304 caused c...

Cross-talk between G protein-coupled receptor (GPCR) and epidermal growth factor receptor (EGFR) signaling systems is widely established in a variety of normal and transformed cell types. Here, we demonstrate that the EGFR transactivation signal requires metalloproteinase cleavage of epidermal growth factor-like growth factor precursors in fibroblasts, ACHN kidney, and TccSup bladder carcinoma ...

BACKGROUND AND AIM S100A4 is a well established marker and mediator of metastatic disease, but the exact mechanisms responsible for the metastasis promoting effects are less well defined. We tested a hypothesis that the S100A4 gene plays a role in the proliferation and invasiveness of human renal cancer cells (RCC) and may be associated with its metastatic spread. MATERIALS AND METHODS The sm...

OBJECTIVE Emerging evidence suggest that the acquisition of epithelial mesenchymal transition (EMT) and induction of cancer stem cell (CSC) or cancer stem-like cell phenotype are interrelated and contribute to tumor recurrence and drug resistance. The aim of this study is to shed light on the relationship between EMT and CSCs by using renal cell carcinoma (RCC) cell line, ACHN and 786-0. METH...

BACKGROUND The use of cell lines isolated from metastases should enable the assessment of peculiar aspects of bone resorbing cytokine expression, as well as angiogenetic activity of renal carcinoma bone metastases. MATERIALS AND METHODS A cell line (CRBM-1990) was isolated from a renal cell carcinoma bone metastasis and compared with the ACHN and Caki-1 established lines. The expression of os...

Silibinin, a flavanone in milk thistle, has been shown effective anticancer effects in various kinds of human tumors. In the present study, we investigated whether and how silibinin induces cell apoptosis in human renal cell carcinoma (RCC) cells. We found that silibinin could markedly inhibit the growth of 786-O and ACHN RCC cells by MTT assay and cell apoptosis by flow cytometric analysis. Mo...

Immunotherapy, consisting mainly of interferon (IFN) therapy, has been used to treat renal cell carcinoma refractory to radiotherapy and chemotherapy ever since IFN was reported to be effective against renal cell carcinoma in 1982. The efficacy of IFN is low, with the response rate being only about 20% and the method and duration of its administration have yet to be established. Using viable tu...