abstractbackgroundcontributes substantially to patient morbidity and mortality.in this study we investigatedthe range and distribution of t-lymphocyte. subsets cd3helper/inducer cell,.th ), cd8: severe immunosuppression occurs after large thermal burn and probably+ (t cells) cd4+ (t+ (t suppressor /cytotoxic cells ,ts/c), cd3+cd4thermal injury.+/cd3+cd8+ ratio, cd19+ (b cells) and cd16+ (nk cel...

background: determination of the division history of t cells in vitro is helpful in the study of effector mechanisms against infections. technique described here uses the intracellular fluorescent label carboxyfluorescein diacetate succinimidyl ester (cfse) to monitor the proliferation. methods: in a cross sectional study, blood samples were collected from 7 volunteers with history of cutaneous...

Background: CD8+ cytotoxic T lymphocytes have been recently divided based on their cytokine expression profile. Objective: To evaluate the percentages of CD8+ lymphocytes and their effector subsets including Tc1, Tc2 and Tc17 in the tumor draining lymph nodes (TDLNs) of patients with breast cancer. Methods: Single cell suspensions were obtained from TDLNs of 42 patients with breast cancer. Stai...

Background: Multiple myeloma (MM) is a malignant plasma cell proliferative disorder with limited immunotherapy treatment because of T cell dysfunction. Objective: To investigate the immunomodulatory function of bone marrow mesenchymal stromal cells (MM-BMSCs) on CD8+ T cells. Methods: Proliferation and cytotoxicity were detected by c...

Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD). Materials and Methods: Male 6-week-old C57BL/6 mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cell...

BACKGROUNDS: Seminoma is frequently observed in human and canine testes especially in cryptorchids. Canine and human seminomas are typically associated with leukocytic infiltration. OBJECTIVES: We aimed to identify the type of lymphocytes that infiltrate to seminomas. METHODS: Tumor infiltrating lymphocytes were evaluated by immunohistological techniques in 5 dogs with diffuse seminoma. Routine...

conclusions the cd4+ and cd8+ tcr vβ gene families undergo clonal expansion in cshb patients, and cd8+ t cells play a major role in the pathogenesis of cshb. moreover, the conserved motifs and limited use of joining region genes observed in the cshb patients of this cohort indicated that similar antigenic epitopes are recognized. results the number of oligoclonal or monoclonal expansion tcr vβ ...

CD8(+) T cells play important roles in anti-tumor immunity but distribution profile or functional characteristics of effector memory subsets during tumor progression are unclear. We found that, in oral squamous carcinoma patients, circulating CD8(+) T cell pools skewed toward effector memory subsets with the distribution frequency of CCR7(-)CD45RA(-)CD8(+) T cells and CCR7(-) CD45RA(+)CD8(+) T ...

background: cd8+ cytotoxic t lymphocytes have been recently divided based on their cytokine expression profile. objective: to evaluate the percentages of cd8+ lymphocytes and their effector subsets including tc1, tc2 and tc17 in the tumor draining lymph nodes (tdlns) of patients with breast cancer. methods: single cell suspensions were obtained from tdlns of 42 patients with breast cancer. stai...

The extent to which the progeny of one primary memory CD8 T cell differs from the progeny of one naive CD8 T cell of the same specificity remains an unresolved question. To explore cell-autonomous functional differences between naive and memory CD8 T cells that are not influenced by differences in the priming environment, an experimental model has been developed in which physiological numbers o...