Microfluidic devices have been used to study high-density cultures of many cell types. Because cell-to-cell signaling is local, however, there exists a need to develop culture systems that sustain small numbers of neurons and enable analyses of the microenvironments. Such cultures are hard to maintain in stable form, and it is difficult to prevent cell death when using primary mammalian neurons...

The method of C. Hallauer and G. Kronauer (Arch. Ges. Virusforsch. 15:433, 1965) for extraction of cell-associated virus by treating infected monolayers with isotonic glycine buffer (pH 9) was applied to human cytomegalovirus. This procedure yields infectious cell-free virus and permits serial extractions of the same infected monolayers with no major damage to the cells. The properties of glyci...

Streptoverticillium fervens produced a new antibiotic, FR-900848, which has a high specific activity against filamentous fungi. Purified by solvent extraction and column chromatography, the compound appears as colorless crystals. Its structure is C32H43N3O6, which consists of 5'-amino-5'-deoxy-5,6-dihydrouridine with an unsaturated fatty acid having unprecedented four serial and one isolated cy...

OBJECTIVE To optimise sample preparation of synovial fluid for Chlamydia trachomatis (CT) specific polymerase chain reaction (PCR). METHODS Serial dilutions of purified CT elementary bodies in synovial fluid were prepared. The synovial fluid pellet was processed by eight different methods of sample preparation. Then samples were analysed by CT specific PCR. The sensitivity of PCR was the basi...

It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.