نتایج جستجو برای: ثبت whole cell patch clamp

تعداد نتایج: 1972952  

2017
Luca A. Annecchino Alexander R. Morris Caroline S. Copeland Oshiorenoya E. Agabi Paul Chadderton Simon R. Schultz

Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed "blind," meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to ...

2012
Manabu Kubokawa

Ion channels are well-known functional proteins, and their activity can be directly estimated by monitoring ion currents through channel proteins using the patch-clamp technique (Hamill et al., 1981), which is a fine tool for the investigation of not only ion channels, but also the membrane current and membrane potential of the whole cell. Many investigators have applied the patch-clamp techniq...

Journal: :Journal of neurophysiology 2016
Qiuyu Wu 吴秋雨 Ilya Kolb Brendan M Callahan Zhaolun Su William Stoy Suhasa B Kodandaramaiah Rachael Neve Hongkui Zeng Edward S Boyden Craig R Forest Alexander A Chubykin

Patch clamp is the main technique for measuring electrical properties of individual cells. Since its discovery in 1976 by Neher and Sakmann, patch clamp has been instrumental in broadening our understanding of the fundamental properties of ion channels and synapses in neurons. The conventional patch-clamp method requires manual, precise positioning of a glass micropipette against the cell membr...

Journal: :Biophysical journal 2002
Niels Fertig Robert H Blick Jan C Behrends

The state of the art technology for the study of ion channels is the patch clamp technique. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets. The most popular (whole cell) variant of the technique detects the ensemble current over the entire cell membrane. Patch clamping is still a laborious process, requiring a skilled expe...

E Cherubini MH Mohajerani

A particular characteristic of the neonatal hippocampus is the presence of spontaneous network-driven oscillatory events, the so-called giant depolarizing potentials (GDPs). GDPs depend on the interplay between GABA and glutamate. Early in development, GABA, acting on GABAA receptors, depolarizes neuronal membranes via a Cl- efflux. Glutamate, via AMPA receptors, generates a positive feedback n...

Journal: :Ultramicroscopy 2008
Evren Pamir Michael George Niels Fertig Martin Benoit

Here we report a new combination of the patch-clamp technique with the atomic force microscope (AFM). A planar patch-clamp chip microstructured from borosilicate glass was used as a support for mechanical probing of living cells. The setup not only allows for immobilizing even a non-adherent cell for measurements of its mechanical properties, but also for simultaneously measuring the electrophy...

2014
Adrien E. Schramm Daniele Marinazzo Thomas Gener Lyle J. Graham

Whole-cell patch recording is an essential tool for quantitatively establishing the biophysics of brain function, particularly in vivo. This method is of particular interest for studying the functional roles of cortical glial cells in the intact brain, which cannot be assessed with extracellular recordings. Nevertheless, a reasonable success rate remains a challenge because of stability, record...

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