BACKGROUND To evaluate the performance of the Abbott RealTime CT/NG assay for detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) among female sex workers (FSWs) in China. METHODS Cervical swabs from 997 participants were blindly detected by the Abbott RealTime CT/NG assay on the automated m2000 molecular platform and Roche Cobas Amplicor CT/NG assay. Discrepant analysis we...

Classical swine fever (CSF) is a contagious and devastating viral disease, causing serious losses in the pig industry worldwide. In the control programmes of CSF, rapid detection and identification of the causative agent is a crucial step. Various PCR based techniques like nested RT-PCR, SYBRGreen based RealTime PCR and TaqMan based Real-time PCR were used for detection of CSFV nucleic acid in ...

Evaluation of Copan FLOQSwab for the molecular detection of Chlamydia trachomatis by Abbott RealTime CT PCR Liselotte Coorevits, Canelle Vanscheeuwijck, Ans Traen*, Luk Bingé, Inge Ryckaert, Elizaveta Padalko Department of Laboratory Medicine, Ghent University Hospital, Belgium, Pasop VZW, Belgium, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Belgium, School ...

Whole Blood: Development and Evaluation of a RealTime PCR Platform, Jens Mueller, Jutta Maria Rox, Katharina Madlener, and Bernd Poetzsch ( Institute for Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany; 2 Department of Haemostaseology and Transfusion Medicine, Kerckhoff-Klinik, Bad Nauheim, Germany; * author for corresponden...

Background: CAPZA3 is one of the actin-capping proteins believed to be play an important role in controlling of actin polymerization during spermiogenesis and has been shown to be peresent in mature sperm. Immunohistochemistry showed that human CAPZA3 is localized in the neck region of ejaculated sperm, with moderate and faint signals also detected in the tail and post-acrosome region respectiv...