Radical Scavenging capacity Abstract: In-vitro antioxidant activity of methanolic extract of Rhinacanthus nasutus leaves was determined by DPPH free radical scavenging assay and Reducing Power assays. Ascorbic acid was used as standard and positive control for both the analysis. All the analysis was made with the use of UV-Visible Spectrophotometer. The methanolic extract of Rhinacanthus nasutu...

Hemp protein hydrolysates with various yields of trichloroacetic acid (TCA)-soluble peptides (Ysp) and surface hydrophobicity (Ho) were obtained by Neutrase ® hydrolysis from hemp protein isolate (HPI). The peptide profiles, amino acid composition and antioxidant activities (DPPH radical scavenging ability, reducing power and Fe chelating ability) of the hydrolysates, obtained at 60–240 min, we...

This work proposes the application of batch-injection analysis with amperometric detection to determine the antioxidant capacity of real samples based on the measurement of DPPH radical consumption. The efficient concentration or EC50 value corresponds to the concentration of sample or standard required to scavenge 50% DPPH radicals. For the accurate determination of EC50, samples were incubate...

Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-...

Radical scavenging and antioxidant activities of nine flavonoids previously isolated from the aerial parts of Chenopodium bonus-henricus L. were established using DPPH and ABTS free radicals as well as inhibition of lipid peroxidation in a linoleic acid system by the ferric thiocyanate method. Patuletin glycosides, compounds 1, 2 and 7 showed the highest DPPH (85.78%, 85.59%, 86.07%) and ABTS a...

Molyneux, P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity Songklanakarin J. Sci. Technol., 2004, 26(2) : 211-219 The use of the stable free radical diphenylpicrylhydrazyl (DPPH) to estimate the activity of antioxidants is reviewed. Current applications of the method are examined, particularly the use of the parameter EC50 (substrate concen...

As plants produce a huge amount of antioxidants they can represent a source of new compounds with antioxidant activities. From this point of view, the main goal of this research was to study the antioxidant activities of the Ficus carica through the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicalscavenging method. Since DPPH assay has been largely used as a quick, reliable, and reproducible parame...

1,1-dipheny-2-picrylhydrazyl (DPPH) scavenging activities of the 80% methanolic leaf extracts of three cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Mai-Men-Dong (Liriope spicata L.) are analyzed by spectrophotometry. The concentrations required for 50% inhibition (IC 50 ) of DPPH radicals were 81.08, 96.97, and 53.78 μg/mL, respectively. The methanolic extracts were further partit...