In vitro genotoxicity assays are often used to compare tobacco smoke particulate matter (PM) from different cigarettes. The quantitative aspect of the comparisons requires appropriate statistical methods and replication levels, to support the interpretation in terms of power and significance. This paper recommends a uniform statistical analysis for the Ames test, mouse lymphoma mammalian cell m...

Since 1996, aging studies using several strains of long-lived mutant mice have been conducted. Among these studies, Ames dwarf mice have been extensively examined to seek clues regarding the role of the growth hormone/insulin-like growth factor-1 axis in the aging process. Interestingly, these projects demonstrate that Ames dwarf mice have physiological characteristics that are similar to those...

We modified the phosphocellulose binding assay for aminoglycoside-modifying enzymes (AMEs) by use of microdilution plates and a multichannel micropipette. Batteries of aminoglycoside substrates for screening organisms for the presence of AMEs as well as for subclassifying enzymes were prepared and stored in microdilution plates. When tested in parallel with the conventional tube reaction assay,...

Hypopituitary Ames dwarf mice have low circulating growth hormone (GH)/IGF-I levels, and they have extended longevity and exhibit many symptoms of delayed aging. To elucidate the vascular consequences of Ames dwarfism we compared endothelial O2(-) and H2O2 production, mitochondrial reactive oxygen species (ROS) generation, expression of antioxidant enzymes, and nitric oxide (NO) production in a...

The aim of the present study was to develop and validate a new instrument to measure empathy and sympathy in adolescents that differentiates between empathy and sympathy, and balances its emphasis on affective and cognitive empathy. The psychometric properties of the Adolescent Measure of Empathy and Sympathy (AMES) were established in two studies. In the first study, among 499 adolescents (10–...

This paper is a summary of the life and major achievements of Adelbert Ames, Jr., an American ophthalmologist and perceptual psychologist, who had initially wanted to be a visual artist. He is most widely remembered today as the inventor of the Ames Demonstrations in Perception, the most famous of which consists of a distorted room in which people seem to shrink as they walk from one corner to ...

The Shapley-Ames Catalog of 1276 galaxies with B < 12.5 is compared with the Sanders et al. all sky sample of the 629 galaxies with 60 μm flux density > 5.24 Jy. The fraction of Shapley-Ames galaxies that are visible in the IR is found to increase from 0.006 for E and E/S0 galaxies to 0.384 for Sc galaxies. The subset of Shapley-Ames galaxies that are detected in the IR has a median blue lumino...

BACKGROUND Semen armeniacae amarum (SAA) is a Chinese traditional medicine and has long been used to control acute lower respiratory tract infection and asthma, as a result of its expectorant and antiasthmatic activities. However, its mutagenicity in vitro and in vivo has not yet been reported. The Ames test for mutagenicity is used worldwide. The histidine contained in biological samples can i...

USDA-ARS Corn Insects and Crop Genetics Research Unit, Ames, IA 50011, Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USDA-ARS Plant Genetics Research Unit and Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USDA-ARS Plant Gene Expression Center, Albany, CA 94710, Department of Molecular and Cell Biology, University of Califo...