Destructive communication is a problem within the NHS; however previous research has focused on bullying. Rude, dismissive and aggressive (RDA) communication between doctors is a more widespread problem and underinvestigated. We conducted a mixed method study combining a survey and focus groups to describe the extent of RDA communication between doctors, its context and subsequent impact. In to...

Forty patients with "rate dependent aberrancy" (RDA) were studied. This large group of patients permitted a clear definition of the syndromne and recognition of a number of features not previously described. These proved to have a significant bearing on the recognition and differential diagnosis of RDA and non-rate dependent aberrancy. It was found that a small change in cycle length, perhaps t...

The difference products (DP) of representational difference analyses (RDA) were used as hybridization probes on cDNA arrays. The effectivity of RDA products obtained with increasing driver/tester ratios (DP 1 = 100:1, DP 2 = 800:1 and DP 3 = 400,000:1) to isolate differentially expressed genes was compared with the effectivity of conventional differential hybridizations. Pacreatic cancer and co...

Helicobacter infection leads to chronic inflammation of the stomach. Although the infection persists in spite of an immune response, animal studies have shown that adjuvant-based oral vaccines can protect against infection and even eliminate established infection. These vaccines are thought to induce a Th2 immune response, counterbalancing the Th1 response seen with natural infections. As a pre...

Today’s rapid development of powerful tools for gene expression analysis provides unprecedented resources for elucidating complex molecular events. The objective of this work has been to apply, combine and evaluate tools for analysis of differential gene expression using atherosclerosis as a model system. First, an optimised solid-phase protocol for representational difference analysis (RDA) wa...

Representational difference analysis (RDA) is a powerful technique for cloning the differences between genomes, and has recently been adapted for cloning differentially expressed genes. RDA, like other PCR-based differential screening methods, is prone to the production of false positives. We have identified a major source of false positives in RDA of cDNA and have introduced improvements which...

Resource Description & Access (RDA) is intended to provide a flexible and extensible framework that can accommodate all types of content and media within rapidly evolving digital environments while also maintaining compatibility with the Anglo-American Cataloguing Rules, 2nd edition (AACR2). The cataloging community is grappling with practical issues in navigating the transition from AACR2 to R...

BACKGROUND The establishment of staff canteens is expected to improve the nutritional situation of Cambodian garment workers. The objective of this study is to assess the nutritive value of low-price model lunch sets provided at a garment factory in Phnom Penh, Cambodia. METHODS Exemplary lunch sets were served to female workers through a temporary canteen at a garment factory in Phnom Penh. ...